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CRISPR-Cas9 系统:一种具有无限可能的基因组编辑工具。

CRISPR-Cas9 system: A genome-editing tool with endless possibilities.

机构信息

Genomic Division, National Institute of Agriculture Science, Rural Development Administration, Jeonju, Republic of Korea.

Department of Soil Science, Acharya Narendra Dev University of Agriculture and Technology, Ayodhya, India.

出版信息

J Biotechnol. 2020 Aug 10;319:36-53. doi: 10.1016/j.jbiotec.2020.05.008. Epub 2020 May 22.

Abstract

The discovery of CRISPR: Cas9 and its application as a powerful gene-editing tool has transformed the world of basic and applied science, especially the molecular biology dome. Also, the smooth, quick, flexible, and very efficient nature of this technology has enabled the biologists to alter the genome of prokaryotes to complex eukaryotic systems, including plants and animals. Using CRISPR and associated tools, investigation, control, and modification of significant biological events have been more accessible than before. These biological scissors are now being used to accelerate breeding programs of crop and livestock, engineer new antimicrobials, and control disease-carrying pathogens. However, like other techniques, these cutters emerged as a double-edged sword and put several challenges to the scientific society. Here in this review article, we summarized the beneficial application of the CRISPR: Cas9 system and unsafe perception to the society if handled carelessly. We also discussed the limitations and ethical issues related to CRISPR: Cas9 technology.

摘要

CRISPR-Cas9 的发现及其作为一种强大的基因编辑工具的应用改变了基础科学和应用科学的世界,尤其是分子生物学领域。此外,这项技术的顺利、快速、灵活和非常高效的特性使生物学家能够改变原核生物的基因组,使其适用于复杂的真核生物系统,包括植物和动物。利用 CRISPR 及其相关工具,调查、控制和修饰重要的生物学事件变得比以往更容易。这些生物剪刀现在正被用于加速作物和牲畜的繁殖计划,设计新的抗生素,并控制携带疾病的病原体。然而,与其他技术一样,这些剪刀也成为了一把双刃剑,给科学界带来了一些挑战。在这篇综述文章中,我们总结了 CRISPR-Cas9 系统的有益应用以及如果不小心处理可能对社会造成的不安全感知。我们还讨论了与 CRISPR-Cas9 技术相关的局限性和伦理问题。

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