缺氧通过HIF-1α/ALKBH5信号通路促进垂体腺瘤增殖。
Hypoxia promotes proliferation of pituitary adenomas by HIF-1α/ALKBH5 signaling .
作者信息
Qian Yuan, Zhang Chao, Wang Wei, Lu Di, Li Junjun, Li Liyan, Li Yao, Qiao Yisheng, Song Hao, Deng Xingli
机构信息
Yunnan Key Laboratory of Laboratory Medicine, 1st Affiliated Hospital of Kunming Medical University Kunming, China.
Department of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Health Hospital Kunming, China.
出版信息
Int J Clin Exp Pathol. 2020 May 1;13(5):1030-1034. eCollection 2020.
Hypoxia is a common phenomenon in pituitary adenomas (PAs). The role and mechanism of hypoxia in the PAs remains elusive. This work aimed to explore the effect of hypoxia on PAs in vitro. PA cells GT1-1 were cultured and treated under hypoxic condition. Cell proliferation assay showed the proliferation of PA cells was increased significantly by hypoxia treatment, with a peak at 12 hours. qPCR and western blot indicated that the expression of HIF-1α, ALKBH5, and Nanog were elevated by hypoxia stimuli. In conclusion, our funding demonstrated that hypoxia could increase Nanog expression through HIF-1α/ALKBH5 signaling, thereby promoting the proliferation of PA cells.
缺氧是垂体腺瘤（PA）中的常见现象。缺氧在PA中的作用和机制仍不清楚。这项工作旨在探讨缺氧对体外PA的影响。将PA细胞GT1-1在缺氧条件下进行培养和处理。细胞增殖试验表明，缺氧处理显著增加了PA细胞的增殖，在12小时达到峰值。定量聚合酶链反应（qPCR）和蛋白质免疫印迹法（western blot）表明，缺氧刺激使缺氧诱导因子-1α（HIF-1α）、RNA去甲基化酶5（ALKBH5）和Nanog的表达升高。总之，我们的研究表明，缺氧可通过HIF-1α/ALKBH5信号通路增加Nanog表达，从而促进PA细胞的增殖。
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