工程化芯片上的微生理免疫系统反应
Engineering Microphysiological Immune System Responses on Chips.
机构信息
Department of Bioengineering, University of Washington, Seattle, WA 98109, USA.
Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.
出版信息
Trends Biotechnol. 2020 Aug;38(8):857-872. doi: 10.1016/j.tibtech.2020.01.003. Epub 2020 Feb 18.
Tissues- and organs-on-chips are microphysiological systems (MPSs) that model the architectural and functional complexity of human tissues and organs that is lacking in conventional cell monolayer cultures. While substantial progress has been made in a variety of tissues and organs, chips recapitulating immune responses have not advanced as rapidly. This review discusses recent progress in MPSs for the investigation of immune responses. To illustrate recent developments, we focus on two cases in point: immunocompetent tumor microenvironment-on-a-chip devices that incorporate stromal and immune cell components and pathomimetic modeling of human mucosal immunity and inflammatory crosstalk. More broadly, we discuss the development of systems immunology-on-a-chip devices that integrate microfluidic engineering approaches with high-throughput omics measurements and emerging immunological applications of MPSs.
组织和器官芯片是微生理系统 (MPS)，可模拟人体组织和器官的结构和功能复杂性，而这在传统的细胞单层培养中是缺乏的。虽然在各种组织和器官中已经取得了相当大的进展，但模拟免疫反应的芯片并没有像预期的那样迅速发展。本文综述了用于研究免疫反应的 MPS 的最新进展。为了说明最近的发展，我们重点介绍两个例子：免疫活性肿瘤微环境芯片设备，其中包含基质和免疫细胞成分，以及人类黏膜免疫和炎症串扰的病理模拟。更广泛地说，我们讨论了系统免疫学芯片设备的发展，该设备将微流控工程方法与高通量组学测量以及 MPS 的新兴免疫学应用相结合。
Zotero 插件