伊朗多囊卵巢综合征女性中（A140D）和（N142D）基因多态性的评估。
Assessment of (A140D) and (N142D) Gene Polymorphisms in Iranian Women with Polycystic Ovarian Syndrome.
作者信息
Miraghaee Seyyed Shahram, Sohrabi Maryam, Jalili Cyrus, Bahrehmand Fariborz
机构信息
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
出版信息
Rep Biochem Mol Biol. 2020 Apr;9(1):8-13. doi: 10.29252/rbmb.9.1.8.
BACKGROUND
Glutathione S-transferases (GSTs) protect cells from oxidative stress (OS). In humans, the GST omega class contains two expressed genes, and . Because OS is involved in the pathogenesis of polycystic ovary syndrome (PCOS), the aim of this study was to investigate the relationship between A140D (rs4925) and N142D (rs156697) polymorphisms in PCOS patients.
METHODS
175 PCOS patients and 161 healthy controls were selected among women in Kermanshah province, Iran. and were genotyped using allele-specific PCR (AS-PCR) and PCR-RFLP, respectively.
RESULTS
For , the DD genotype and the D allele led to 2.17- (= 0.02) and 1.5-fold (P= 0.01) increases, respectively, in the odds ratios for PCOS. No significant difference was found between control and patient groups for the N142D genotype or allele frequency. GSTO1 and GSTO2 genotype interaction analysis showed that individuals with the AD or DD genotypes and the GSTO2 NN or DN genotypes had a 1.53-fold (= 0.007) increase in PCOS risk over AA and GSTO2 DD individuals.
CONCLUSION
The A140D polymorphism is a risk factor for PCOS.
背景
谷胱甘肽S-转移酶（GSTs）可保护细胞免受氧化应激（OS）的影响。在人类中，GSTω亚类包含两个表达基因，即 和 。由于氧化应激参与多囊卵巢综合征（PCOS）的发病机制，本研究旨在探讨PCOS患者中 A140D（rs4925）和 N142D（rs156697）多态性之间的关系。
方法
在伊朗克尔曼沙阿省的女性中选取了175例PCOS患者和161例健康对照。分别采用等位基因特异性PCR（AS-PCR）和PCR-RFLP对 和 进行基因分型。
结果
对于 ，DD基因型和D等位基因使PCOS的比值比分别增加了2.17倍（ = 0.02）和1.5倍（P = 0.01）。在 N142D基因型或等位基因频率方面，对照组和患者组之间未发现显著差异。GSTO1和GSTO2基因型交互分析显示，与 AA和GSTO2 DD个体相比，具有 AD或DD基因型以及GSTO2 NN或DN基因型的个体患PCOS的风险增加了1.53倍（ = 0.007）。
结论
A140D多态性是PCOS的一个风险因素。
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