一种用于水溶液中汞离子检测的基于反应的比率荧光探针。
A reaction-based ratiometric fluorescent probe for mercury ion detection in aqueous solution.
作者信息
Liu Shudi, Feng Di, Zhang Liangwei, Song He, Wang Yue, Zhang Xia, Zhao Qingjun, Chen Lingxin
机构信息
College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, PR China.
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Dec 15;243:118817. doi: 10.1016/j.saa.2020.118817. Epub 2020 Aug 11.
Mercury ions are crucially harmful to ecosystem and human being due to their characters of bioaccumulation and difficulty of biochemical degradation. Therefore, development of mercury ion detection methods has attracted increasing interests recently. In this study, we successfully synthesized a hydroxyphenylbenzothiazole (HBT)-based fluorescent probe HBT-Hg in an extremely simple manner for mercuric ions detection. The spectral studies revealed that the probe HBT-Hg could react with Hg selectively and sensitively in PBS buffer (10 mM, pH = 7.40), showing ratiometric fluorescent changes from blue to light green. The response mechanism of the probe HBT-Hg and Hg was finally confirmed by HPLC analysis, viz., the probe HBT-Hg converted to its precursor compound 1. Finally, the probe HBT-Hg was successfully applied in monitoring Hg in living A549 cells.
汞离子因其生物累积性和生化降解困难的特性，对生态系统和人类具有极其严重的危害。因此，近年来汞离子检测方法的开发引起了越来越多的关注。在本研究中，我们以极其简单的方式成功合成了一种基于羟基苯基苯并噻唑（HBT）的荧光探针HBT-Hg用于汞离子检测。光谱研究表明，探针HBT-Hg能在PBS缓冲液（10 mM，pH = 7.40）中与汞选择性且灵敏地反应，呈现出从蓝色到浅绿色的比率荧光变化。通过高效液相色谱分析最终证实了探针HBT-Hg与汞的响应机制，即探针HBT-Hg转化为其前体化合物1。最后，探针HBT-Hg成功应用于监测活的A549细胞中的汞。
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