利用辣根过氧化物酶/磷酸铜杂化纳米花进行过氧化氢的紫外可见检测。
UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers.
机构信息
Institute of Hybrid Materials, National Center of International Joint Research for Hybrid Materials Technology, National Base of International Science & Technology Cooperation, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, People's Republic of China.
Institute of Hybrid Materials, National Center of International Joint Research for Hybrid Materials Technology, National Base of International Science & Technology Cooperation, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, People's Republic of China.
出版信息
Enzyme Microb Technol. 2020 Oct;140:109620. doi: 10.1016/j.enzmictec.2020.109620. Epub 2020 Jun 7.
A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (HO). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu(PO)) as the organic and inorganic components, respectively. HRP/Cu(PO) hybrid nanoflowers (HRP-HNFs) showed linear HO detection range from 100 nM to 100 μM, moreover, HRP-HNFs exhibited good selectivity for HO detection due to the specificity of HRP. In addition, HRP-HNFs have good reusability, excellent color stability and long-term storage stability, which indicate HRP-HNFs are promising for catalysis and biomedical applications.
基于酶-无机杂化纳米花的比色平台被用于检测过氧化氢（HO）的紫外可见检测。通过在室温下孵育辣根过氧化物酶（HRP）和磷酸铜（Cu（PO））分别作为有机和无机成分来制备杂化纳米花。HRP/Cu（PO）杂化纳米花（HRP-HNFs）显示出从 100 nM 到 100 μM 的线性 HO 检测范围，此外，由于 HRP 的特异性，HRP-HNFs 对 HO 的检测表现出良好的选择性。此外，HRP-HNFs 具有良好的可重复使用性、优异的颜色稳定性和长期储存稳定性，这表明 HRP-HNFs在催化和生物医学应用方面具有广阔的应用前景。
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