两种用于特异性识别HClO/NaHSO的咪唑类荧光探针的设计、合成及其应用。
The design and synthesis of two imidazole fluorescent probes for the special recognition of HClO/NaHSO and their applications.
作者信息
Ding Hong-Lin, Pu Yan-Qing, Ye Da-Ying, Dong Zi-Yue, Yang Man, Lü Cheng-Wei, An Yue
机构信息
College of Chemistry and Chemical Engineering, Liaoning Normal University, Huanghe Road 850#, Dalian 116029, P. R. China.
出版信息
Anal Methods. 2020 May 21;12(19):2476-2483. doi: 10.1039/d0ay00334d.
Two fluorescent probes (L1 and L2) based on an imidazole unit were synthesized for the specific detection of ClO- and HSO3-. Density functional theory (DFT) calculations were used to assist in designing the probes. As predicted, L1 could be used to detect ClO- in real water samples and in living cells. It was shown to be a quenching probe. L2 could be used to monitor HSO3- in living cells and is an enhanced fluorescence probe. Further details of the fluorescence recognition mechanism were obtained via HRMS analysis. Moreover, both fluorescent probes showed relatively low detection limits (0.96 and 0.59 μM, respectively), and fast and highly selective fluorescence responses.
合成了两种基于咪唑单元的荧光探针（L1和L2），用于特异性检测ClO-和HSO3-。采用密度泛函理论（DFT）计算辅助设计探针。如预期的那样，L1可用于检测实际水样和活细胞中的ClO-。它是一种猝灭型探针。L2可用于监测活细胞中的HSO3-，是一种增强型荧光探针。通过高分辨质谱（HRMS）分析获得了荧光识别机制的更多细节。此外，两种荧光探针均显示出相对较低的检测限（分别为0.96和0.59 μM），以及快速且高度选择性的荧光响应。
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