使用 Ca/P-PILP 仿生再矿化人工龋牙本质病变。

Biomimetic remineralization of artificial caries dentin lesion using Ca/P-PILP.

机构信息

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China; KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals Leuven Dentistry, Leuven, Belgium.

出版信息

Dent Mater. 2020 Nov;36(11):1397-1406. doi: 10.1016/j.dental.2020.08.017. Epub 2020 Sep 22.

DOI:10.1016/j.dental.2020.08.017

PMID:32977980

Abstract

OBJECTIVE

To assess the ultrastructural change of demineralized dentin collagen during calcium phosphate polymer-induced liquid precursor (Ca/P-PILP) mediated remineralization process and to evaluate the biomimetic remineralization potential of high concentration Ca/P-PILP at demineralized artificial caries dentin lesion, additionally to investigate the bond interfacial integrity as well as the bonding strength of the biomimetic remineralized artificial caries dentin lesion.

METHODS

Demineralized dentin collagen of 5 μm thick was biomimetically remineralized with low, medium concentration Ca/P-PILP for 10 days and high concentration Ca/P-PILP for 10, 15, 20 days. Artificial caries dentin lesion at a thickness of 150 ± 50 μm was biomimetically remineralized with high concentration Ca/P-PILP for 20 days. The biomimetic remineralization of demineralized dentin collagen was observed by scanning electron microscopy (SEM). The biomimetic remineralization intensity and depth of artificial caries dentin lesion was assessed by Electron Probe Micro Analyzer (EPMA). The bonding interfacial integrity between remineralized artificial caries dentin and composite resin was observed by Swept-source optical coherence tomography (SS-OCT) and the bonding strength of remineralized artificial caries dentin was evaluated by micro-tensile bond strength analysis (μTBS).

RESULTS

Solely PAA-PASP solution and solely saturated Ca/P solution can't achieve dentin collagen remineralization. Increased concentration of Ca/P-PILP and prolonged remineralization time can enhance the biomimetic remineralization intensity of demineralized dentin collagen. After treating with high concentration Ca/P-PILP, a 150 ± 50 μm thick layer of demineralized artificial caries dentin lesion was not fully remineralized, and the biomimetic remineralization intensity reached up to 88.0%. Furthermore, a better bonding interfacial integrity with less microgap and increased bond strength at both baseline level and aging level were observed when artificial caries dentin lesion was biomimetically remineralized with high concentration Ca/P-PILP.

SIGNIFICANCE

Biomimetic remineralization of demineralized caries dentin lesion promotes its clinical properties for resin composited adhesive restoration.

摘要

目的

评估脱矿牙本质胶原在磷酸钙聚合物诱导的液体前体（Ca/P-PILP）介导的再矿化过程中的超微结构变化，并评估高浓度 Ca/P-PILP 在脱矿人工龋损牙本质病变中的仿生再矿化潜力，同时研究仿生再矿化人工龋损牙本质的粘结界面完整性和粘结强度。

方法

用低浓度、中浓度 Ca/P-PILP 分别对 5μm 厚的脱矿牙本质胶原仿生再矿化 10 天，用高浓度 Ca/P-PILP 仿生再矿化 10、15、20 天。用高浓度 Ca/P-PILP 对厚度为 150±50μm 的人工龋损牙本质病变仿生再矿化 20 天。用扫描电子显微镜（SEM）观察脱矿牙本质胶原的仿生再矿化情况。用电探针微分析（EPMA）评估人工龋损牙本质的仿生再矿化强度和深度。用扫频源光学相干断层扫描（SS-OCT）观察再矿化人工龋损牙本质与复合树脂之间的粘结界面完整性，用微拉伸粘结强度分析（μTBS）评估再矿化人工龋损牙本质的粘结强度。

结果

单独的 PAA-PASP 溶液和单独的饱和 Ca/P 溶液不能实现牙本质胶原的再矿化。增加 Ca/P-PILP 的浓度和延长再矿化时间可以增强脱矿牙本质胶原的仿生再矿化强度。用高浓度 Ca/P-PILP 处理后，150±50μm 厚的脱矿人工龋损牙本质病变未完全再矿化，仿生再矿化强度达到 88.0%。此外，当用高浓度 Ca/P-PILP 对人工龋损牙本质病变进行仿生再矿化时，观察到粘结界面完整性更好，微间隙更少，在基线水平和老化水平的粘结强度更高。