用于严重急性呼吸综合征冠状病毒2（SARS-CoV-2）全基因组分析的靶向文库制备解决方案的性能
Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis.
作者信息
Klempt Petr, Brož Petr, Kašný Martin, Novotný Adam, Kvapilová Kateřina, Kvapil Petr
机构信息
Institute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic.
Department of Biology and Medical Genetics, Second Faculty of Medicine, Charles University and Motol University Hospital, V Úvalu 84, 150 06 Prague, Czech Republic.
出版信息
Diagnostics (Basel). 2020 Sep 29;10(10):769. doi: 10.3390/diagnostics10100769.
Single next-generation sequencing (NGS) proved to be an important tool for monitoring the SARS-CoV-2 outbreak at the global level Until today, thousands of SARS-CoV-2 genome sequences have been published at GISAID (Global Initiative on Sharing All Influenza Data) but only a portion are suitable for reliable variant analysis. Here we report on the comparison of three commercially available NGS library preparation kits. We discuss advantages and limitations from the perspective of required input sample quality and data quality for advanced SARS-CoV-2 genome analysis.
事实证明，单链下一代测序（NGS）是在全球范围内监测新冠病毒（SARS-CoV-2）疫情的一项重要工具。截至目前，已有数千条SARS-CoV-2基因组序列在全球共享流感数据倡议组织（GISAID）上发布，但只有一部分适合进行可靠的变异分析。在此，我们报告了三种市售NGS文库制备试剂盒的比较情况。我们从先进的SARS-CoV-2基因组分析所需的输入样本质量和数据质量的角度，讨论了其优缺点。
Zotero 插件