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利用肠杆菌基因间重复一致序列聚合酶链反应(ERIC-PCR)对从牛奶、水和肉类样品中分离出的[具体菌株名称缺失]和[具体菌株名称缺失]进行遗传多样性的分子测定。

Molecular determination of genetic diversity among and isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR).

作者信息

Igwaran Aboi, Okoh Anthony Ifeanyi

机构信息

SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, South Africa.

Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa.

出版信息

Infect Ecol Epidemiol. 2020 Oct 6;10(1):1830701. doi: 10.1080/20008686.2020.1830701.

Abstract

Consumption of contaminated meat, milk, and water are among the major routes of human campylobacteriosis. This study aimed to determined the genetic diversity of and isolated from meat, milk, and water samples collected from different locations. From the 376 samples (meat = 248, cow milk = 72, and water = 56) collected, a total of 1238 presumptive isolates were recovered and the presence of the genus were detected in 402 isolates, and from which, 85 and 67 isolates were identified as and respectively. Of which, 71 isolates identified as (n = 35) and (n = 36) were randomly selected from meat, milk, and water samples and were genotyped using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The digital images of the ERIC-PCR genotype were analyzed by GelJ v.2.0 software. The diversity and similarity of the isolates were assessed via an unweighted-pair group method using average linkages clustering algorithm. The results showed that the 36  strains separated into 29 ERIC-genotypes and 4 clusters while the 35  were delineated into 29 ERIC-genotypes and 6 clusters. The study revealed the genetic diversity among and strains recovered from different matrices characterized by Gelj.

摘要

食用受污染的肉类、牛奶和水是人类弯曲杆菌病的主要传播途径。本研究旨在确定从不同地点采集的肉类、牛奶和水样中分离出的空肠弯曲菌和结肠弯曲菌的遗传多样性。在所采集的376份样本(肉类=248份、牛奶=72份、水=56份)中,共分离出1238株疑似弯曲菌,在402株分离物中检测到弯曲菌属的存在,其中分别有85株和67株分离物被鉴定为空肠弯曲菌和结肠弯曲菌。其中,从肉类、牛奶和水样中随机选取71株被鉴定为空肠弯曲菌(n = 35)和结肠弯曲菌(n = 36)的菌株,使用肠杆菌重复基因间共识PCR(ERIC-PCR)进行基因分型。通过GelJ v.2.0软件分析ERIC-PCR基因型的数字图像。采用平均连锁聚类算法的非加权配对组方法评估分离物的多样性和相似性。结果显示,36株结肠弯曲菌分为29个ERIC基因型和4个簇,而35株空肠弯曲菌分为29个ERIC基因型和6个簇。该研究揭示了通过Gelj表征的从不同基质中分离出的空肠弯曲菌和结肠弯曲菌菌株之间的遗传多样性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae94/7580821/71d8c4fbe8ac/ZIEE_A_1830701_F0001_OC.jpg

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