血清蛋白-透明质酸复合纳米载体：结构特征与包封可能性。

Serum protein-hyaluronic acid complex nanocarriers: Structural characterisation and encapsulation possibilities.

机构信息

Department of Physical Chemistry and Materials Science, University of Szeged, H-6720, Rerrich B. Square 1, Szeged, Hungary.

Department of Physical Chemistry and Materials Science, University of Szeged, H-6720, Rerrich B. Square 1, Szeged, Hungary; MTA-SZTE Biomimetic Systems Research Group, Department of Medical Chemistry, Faculty of Medicine, University of Szeged, H-6720 Dóm Square 8, Szeged, Hungary.

出版信息

Carbohydr Polym. 2021 Jan 1;251:117047. doi: 10.1016/j.carbpol.2020.117047. Epub 2020 Sep 7.

DOI:10.1016/j.carbpol.2020.117047

PMID:33142605

Abstract

A protein-polysaccharide-based potential nanocarrier system have been developed via a simple, one-step preparation protocol without the use of long-term heating and the utilization of hardly removable crosslinking agents, surfactants, and toxic organic solvents. To the best of our knowledge, this article is the first which summarizes in detail the pH-dependent quantitative relationship between the bovine serum albumin (BSA) and hyaluronic acid (HyA) confirmed by several physico-chemical techniques. The formation of colloidal complex nanoconjugates with average diameter of ca. 210-240 nm is strongly depend on the pH and the applied BSA:HyA mass ratio. Particle charge titrations studies strongly support the core-shell type structure, where the BSA core is covered by a thick HyA shell. Besides the optimization of these conditions, the drug encapsulation capacity and the dissolution profiles have been also studied for ibuprofen (IBU) and 2-picolinic acid (2-PA) as model drugs.

摘要

已开发出一种基于蛋白质-多糖的潜在纳米载体系统，该系统通过简单的一步制备方案制备，无需使用长期加热和难以去除的交联剂、表面活性剂和有毒有机溶剂。据我们所知，本文是第一篇详细总结牛血清白蛋白（BSA）和透明质酸（HyA）之间的 pH 值依赖性定量关系的文章，该关系已通过几种物理化学技术得到证实。平均直径约为 210-240nm 的胶体复合纳米复合物的形成强烈依赖于 pH 值和所施加的 BSA:HyA 质量比。颗粒电荷滴定研究强烈支持核壳型结构，其中 BSA 核被厚厚的 HyA 壳覆盖。除了优化这些条件外，还研究了布洛芬（IBU）和 2-吡啶甲酸（2-PA）作为模型药物的药物包封能力和溶解曲线。