调控 Aub-TDRD 相互作用阐明了 piRNA 的扩增和种质形成。
Modulation of Aub-TDRD interactions elucidates piRNA amplification and germplasm formation.
机构信息
Division of Neuropathology, Departments of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Laboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.
出版信息
Life Sci Alliance. 2020 Dec 29;4(3). doi: 10.26508/lsa.202000912. Print 2021 Mar.
Aub guided by piRNAs ensures genome integrity by cleaving retrotransposons, and genome propagation by trapping mRNAs to form the germplasm that instructs germ cell formation. Arginines at the N-terminus of Aub (Aub-NTRs) interact with Tudor and other Tudor domain-containing proteins (TDRDs). Aub-TDRD interactions suppress active retrotransposons via piRNA amplification and form germplasm via generation of Aub-Tudor ribonucleoproteins. Here, we show that Aub-NTRs are dispensable for primary piRNA biogenesis but essential for piRNA amplification and that their symmetric dimethylation is required for germplasm formation and germ cell specification but largely redundant for piRNA amplification.
Aub 通过切割逆转录转座子来确保基因组完整性，通过捕获 mRNA 形成种质来指导生殖细胞的形成，从而促进基因组的传播。Aub（Aub-NTRs）的 N 端精氨酸与 Tudor 和其他含有 Tudor 结构域的蛋白（TDRDs）相互作用。Aub-TDRD 相互作用通过 piRNA 扩增抑制活性逆转录转座子，并通过生成 Aub-Tudor 核糖核蛋白形成种质。在这里，我们表明 Aub-NTRs 对于初级 piRNA 的生物发生不是必需的，但对于 piRNA 的扩增是必需的，并且它们的对称二甲基化对于种质的形成和生殖细胞的特化是必需的，但对于 piRNA 的扩增则是冗余的。
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