基于多孔 ZnO-CoO 纳米笼的 MOF 衍生纳米酶用于测定淀粉样β肽的比色法。

Colorimetric determination of amyloid-β peptide using MOF-derived nanozyme based on porous ZnO-CoO nanocages.

机构信息

College of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, China.

Department of Chemistry, School of Sciences, Hebei University of Science and Technology, Shijiazhuang, 050018, China.

出版信息

Mikrochim Acta. 2021 Jan 27;188(2):56. doi: 10.1007/s00604-021-04705-4.

DOI:10.1007/s00604-021-04705-4

PMID:33502585

Abstract

A sensitive and rapid colorimetric biosensor has been developed for determination of amyloid-β peptide (Aβ) and study of amyloidogenesis based on the high peroxidase-like activity of porous bimetallic ZnO-CoO nanocages (NCs). Due to the high binding ability of Aβ monomer to ZnO-CoO NCs, the catalytic activity of ZnO-CoO NCs can be significantly suppressed by Aβ monomer. This finding forms the basis for a colorimetric assay for Aβ monomer detection. The detection limit for Aβ monomer is 3.5 nM with a linear range of 5 to 150 nM (R = 0.997). The system was successfully applied to the determination of Aβ monomer in rat cerebrospinal fluid. Critically, the different inhibition effects of monomeric and aggregated Aβ species on the catalytic activity of ZnO-CoO NCs enabled the sensor to be used for tracking the dynamic progress of Aβ aggregation and screening Aβ inhibitors. Compared with the commonly used thioflavin T fluorescence assay, this method provided higher sensitivity to the formation of Aβ oligomer at the very early assembly stage. Our assay shows potential application in early diagnosis and therapy of Alzheimer's disease (AD).

摘要

一种灵敏、快速的比色生物传感器已被开发出来，用于基于多孔双金属 ZnO-CoO 纳米笼（NCs）的高过氧化物酶样活性来测定淀粉样β肽（Aβ）和研究淀粉样蛋白的形成。由于 Aβ 单体与 ZnO-CoO NCs 具有高结合能力，Aβ 单体可以显著抑制 ZnO-CoO NCs 的催化活性。这一发现为 Aβ 单体的比色检测分析奠定了基础。Aβ 单体的检测限为 3.5 nM，线性范围为 5 至 150 nM（R = 0.997）。该系统成功应用于大鼠脑脊液中 Aβ 单体的测定。重要的是，单体和聚集态 Aβ 物种对 ZnO-CoO NCs 催化活性的不同抑制作用使传感器能够用于跟踪 Aβ 聚集的动态进展并筛选 Aβ 抑制剂。与常用的硫黄素 T 荧光测定法相比，该方法对 Aβ 低聚物在早期组装阶段的形成具有更高的灵敏度。我们的测定方法在阿尔茨海默病（AD）的早期诊断和治疗中显示出潜在的应用价值。