利用重组谷氨酸棒杆菌从甘露醇高效生物合成 l-鸟氨酸。
Highly efficient biosynthesis of l-ornithine from mannitol by using recombinant Corynebacterium glutamicum.
机构信息
College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Jiangxi Agricultural University, Nanchang 330045, China.
College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Jiangxi Agricultural University, Nanchang 330045, China.
出版信息
Bioresour Technol. 2021 May;327:124799. doi: 10.1016/j.biortech.2021.124799. Epub 2021 Feb 1.
Mannitol is a promising six-carbon sugar alcohol that is widely found in macroalgae. The potential of mannitol as a renewable raw material is of interest due to the advantages of ocean farms. Herein, the biobased production of l-ornithine from mannitol was resoundingly demonstrated for the first time in engineered Corynebacterium glutamicum S9114 through the deletion of the mannitol repressor MtlR. By modulating the expression of mtlD and reinforcing the fructose metabolic pathway, we generated the strain MTL13 that produced 54.56 g/L of l-ornithine with a yield of 0.47 g/g on mannitol. These results illustrate the robust conversion from mannitol to l-ornithine using engineered Corynebacterium glutamicum, providing a reference for the biobased production of additional chemicals from mannitol.
甘露醇是一种有前景的六碳糖醇，广泛存在于大型藻类中。由于海洋农场的优势，甘露醇作为可再生原料的潜力引起了人们的兴趣。在此，通过敲除甘露醇阻遏物 MtlR，首次在工程化谷氨酸棒杆菌 S9114 中响亮地证明了从甘露醇生物合成 l-鸟氨酸。通过调节 mtlD 的表达并加强果糖代谢途径，我们生成了 MTL13 菌株，该菌株在甘露醇上生产 54.56 g/L 的 l-鸟氨酸，产率为 0.47 g/g。这些结果说明了使用工程化谷氨酸棒杆菌从甘露醇到 l-鸟氨酸的稳健转化，为从甘露醇生物合成其他化学品提供了参考。
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