Li Li, Yi Ping, Li Changbao, Xin Ming, Sun Jian, He Xuemei, Sheng Jinfeng, Zhou Zhugui, Zheng Fengjin, Li Jiemin, Liu Guoming, Ling Dongning, Tang Jie, Li Zhichun, Yang Ying, Tang Yayuan
Agro-food Science and Technology Research Institute Guangxi Academy of Agricultural Sciences Nanning China.
Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology Nanning China.
Food Sci Nutr. 2021 Jan 8;9(2):888-899. doi: 10.1002/fsn3.2052. eCollection 2021 Feb.
Fresh-cut lettuce has a short shelf-life due to enzymatic browning and oxidative senescence. The present study investigated effects of polysaccharide-based edible coatings (alginate, chitosan, and carrageenan) on enzymatic browning and antioxidant defense system of fresh-cut lettuces during cold storage (4°C) for 15 days. The results showed that three coatings could inhibit enzymatic browning through maintaining total phenolics (TP) content and decreasing polyphenol oxidase (PPO) and phenylalanine ammonialyase (PAL) activities. These coatings also reduced phospholipase D (PLD) and lipoxygenase (LOX) activities, lowered malondialdehyde (MDA) content, and enhanced antioxidant enzymes (superoxide dismutase, SOD; peroxidase, POD; catalase, CAT; ascorbate peroxidase, APX) activities. Besides, all coatings positively affected sensory properties of fresh-cut lettuces after 3 days storage. Additionally, among three coating treatments, chitosan coating had the most positive effects on quality of fresh-cut lettuce and was the most suitable coating for retarding enzymatic browning and alleviating membrane oxidative damage. These results indicated that polysaccharide-based edible coatings were helpful to maintain quality, inhibit enzymatic browning, and postpone senescence of fresh-cut lettuce.
鲜切生菜由于酶促褐变和氧化衰老而货架期较短。本研究调查了基于多糖的可食性涂层(海藻酸盐、壳聚糖和卡拉胶)对鲜切生菜在4℃冷藏15天期间酶促褐变和抗氧化防御系统的影响。结果表明,三种涂层可通过维持总酚(TP)含量、降低多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性来抑制酶促褐变。这些涂层还降低了磷脂酶D(PLD)和脂氧合酶(LOX)活性,降低了丙二醛(MDA)含量,并增强了抗氧化酶(超氧化物歧化酶,SOD;过氧化物酶,POD;过氧化氢酶,CAT;抗坏血酸过氧化物酶,APX)活性。此外,所有涂层在储存3天后对鲜切生菜的感官特性都有积极影响。另外,在三种涂层处理中,壳聚糖涂层对鲜切生菜品质的积极影响最大,是抑制酶促褐变和减轻膜氧化损伤的最合适涂层。这些结果表明,基于多糖的可食性涂层有助于保持鲜切生菜的品质、抑制酶促褐变并延缓衰老。