淀粉酶可提高废鸡、牛、猪和罗非鱼皮中低分子量胶原蛋白肽的产量。
Amylase enhances production of low molecular weight collagen peptides from the skin of spent hen, bovine, porcine, and tilapia.
机构信息
Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
出版信息
Food Chem. 2021 Aug 1;352:129355. doi: 10.1016/j.foodchem.2021.129355. Epub 2021 Feb 20.
Low molecular weight (LMW) collagen peptides show skin and bone health benefits for human. However, the production of LMW collagen peptides from land vertebrate sources remains challenging due to the presence of advanced glycation end products (AGEs) cross-links. In this study, the effect of α-amylase pre-treatment on proteolytic production of LMW collagen peptides by papain was investigated; spent hen, bovine, porcine, and tilapia skin collagens (HSC, BSC, PSC, and TSC, respectively) were chosen. Results showed that pre-treatment with α-amylase considerably improved the production of LMW peptides (<2 kDa) from HSC (33.79-67.66%), PSC (86.03-90.85%), BSC (6.60-28.78%), and TSC (89.92-90.27%). The HSC presented the highest carbohydrate content and was increased the most in LMW peptides after amylase pretreatment. These results suggested that α-amylase could cleave glycosidic bonds of AGEs between collagen and thus enhance the production of LMW collagen peptides.
低分子量（LMW）胶原蛋白肽对人类的皮肤和骨骼健康有益。然而，由于存在高级糖基化终产物（AGEs）交联，从陆地脊椎动物来源生产 LMW 胶原蛋白肽仍然具有挑战性。在这项研究中，研究了α-淀粉酶预处理对木瓜蛋白酶酶解生产 LMW 胶原蛋白肽的影响；选择了废鸡、牛、猪和罗非鱼皮胶原蛋白（HSC、BSC、PSC 和 TSC，分别）。结果表明，α-淀粉酶预处理可显著提高 HSC（33.79-67.66%）、PSC（86.03-90.85%）、BSC（6.60-28.78%）和 TSC（89.92-90.27%）中 LMW 肽（<2 kDa）的产量。HSC 具有最高的碳水化合物含量，并且在经过淀粉酶预处理后，LMW 肽的含量增加最多。这些结果表明，α-淀粉酶可以切断胶原蛋白中 AGEs 的糖苷键，从而提高 LMW 胶原蛋白肽的产量。
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