Ahp2 是. 的一种裂解噬菌体，其分子特征。
Molecular Characterization of Ahp2, a Lytic Bacteriophage of .
机构信息
Laboratory of Microbial Genetics, Institute of Medical Sciences, Tzu Chi University, No. 701, Sec. 3, Zhongyang Rd., Hualien 97004, Taiwan.
Department of Microbiology, School of Medicine, Tzu Chi University, No. 701, Sec. 3, Zhongyang Rd., Hualien 97004, Taiwan.
出版信息
Viruses. 2021 Mar 14;13(3):477. doi: 10.3390/v13030477.
is an opportunistic pathogen that infects fish, amphibians, mammals, and humans. This study isolated a myophage, vB_AhyM_Ahp2 (Ahp2), that lytically infects . We observed that 96% of the Ahp2 particles adsorbed to within 18 min. Ahp2 also showed a latent period of 15 min with a burst size of 142 PFU/cell. This phage has a linear double-stranded DNA genome of 47,331 bp with a GC content of 57%. At least 20 Ahp2 proteins were detected by SDS-polyacrylamide gel electrophoresis; among them, a 40-kDa protein was predicted as the major capsid protein. Sequence analysis showed that Ahp2 has a genome organization closely related to a group of phages (13AhydR10RR, 14AhydR10RR, 85AhydR10RR, phage 3, 32 Asp37, 59.1), which infect and . The tail module encompassing ORF27-29 in the Ahp2 genome was present in all phages analyzed in this study and likely determines the host range of the virus. This study found that Ahp2 completely lyses AH300206 in 3.5 h at a MOI of 0.0001 and does not lysogenize its host. Altogether, these findings show that Ahp2 is a lytic phage and could be a candidate for therapeutic phage cocktails.
是一种机会性病原体，可感染鱼类、两栖类、哺乳动物和人类。本研究分离到一株裂解性噬菌，vB_AhyM_Ahp2（Ahp2），可感染 。我们观察到，96%的 Ahp2 颗粒在 18 分钟内吸附到 上。Ahp2 的潜伏期为 15 分钟，爆发量为 142PFU/细胞。该噬菌体具有线性双链 DNA 基因组，大小为 47331bp，GC 含量为 57%。通过 SDS-聚丙烯酰胺凝胶电泳检测到至少 20 种 Ahp2 蛋白；其中，预测 40kDa 蛋白为主要衣壳蛋白。序列分析表明，Ahp2 的基因组组织与一组 噬菌体（13AhydR10RR、14AhydR10RR、85AhydR10RR、噬菌体 3、32Asp37、59.1）密切相关，这些噬菌体感染 和 。Ahp2 基因组中 ORF27-29 包含的尾部模块存在于本研究分析的所有 噬菌体中，可能决定了病毒的宿主范围。本研究发现，Ahp2 在 MOI 为 0.0001 时，3.5 小时内可完全裂解 AH300206，且不裂解其宿主。综上所述，这些发现表明 Ahp2 是一种裂解性噬菌体，可作为治疗性噬菌体鸡尾酒的候选物。
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