EasyCloneYALI：用于脂肪假丝酵母 CRISPR 介导的整合和缺失的工具箱。
EasyCloneYALI: Toolbox for CRISPR-Mediated Integrations and Deletions in Yarrowia lipolytica.
机构信息
The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark, Copenhagen, Denmark.
出版信息
Methods Mol Biol. 2021;2307:41-68. doi: 10.1007/978-1-0716-1414-3_3.
In order to unlock the full potential of Yarrowia lipolytica, as model organism and production host, simple and reliable tools for genome engineering are essential. In this chapter, the practical details of working with the EasyCloneYALI Toolbox are described.Highlights of the EasyCloneYALI Toolbox are high genome editing efficiencies, multiplexed Cas9-mediated knockouts, targeted genomic integrations into characterized intergenic loci, as well as streamlined and convenient cloning for both marker-based and marker-free integrative expression vectors.
为了充分挖掘酿酒酵母（Yarrowia lipolytica）这一模式生物和生产宿主的潜力，简单可靠的基因组工程工具必不可少。本章详细介绍了 EasyCloneYALI 工具盒的使用方法。EasyCloneYALI 工具盒的突出特点是基因组编辑效率高、Cas9 介导的多位点敲除、靶向整合到特征性基因间区、以及基于标记和无标记整合表达载体的简化和方便克隆。
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