characterization of pharmaco-eeG fingerprint and sleep-wake profiles of Lavandula angustifolia Mill. essential oil inhalation and diazepam administration in rats
Characterization of pharmaco-EEG fingerprint and sleep-wake profiles of Lavandula angustifolia Mill. essential oil inhalation and diazepam administration in rats.
机构信息
Division of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani Campus, Thailand, Pattani, 94000, Thailand; Biosignal Research Center for Health, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Biosignal Research Center for Health, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
出版信息
J Ethnopharmacol. 2021 Aug 10;276:114193. doi: 10.1016/j.jep.2021.114193. Epub 2021 May 8.
ETHNOPHARMACOLOGICAL RELEVANCE
Lavandula angustifolia Mill. Essential oil (Lavender EO) has a long history of medicinal use and is particularly claimed to possess anxiolytic and sedative properties. Lavender EO aromatherapy has been used to reduce distress and improve insomnia naturally. Increasing evidence appeared to show similarities between the effects of lavender EO and the anxiolytic drugs, benzodiazepines. However, its effects on sleep-wake and electrical brain patterns in comparison to that of the standard anxiolytic, diazepam, remained to be explored.
AIM OF THE STUDY
The aim of this work was to investigate electroencephalography (EEG) profiles and sleep-pattern elicited by lavender EO inhalation compared to that of diazepam, a standard anxiolytic drug in in vivo rat model.
MATERIALS AND METHODS
Adult male Wistar rats were anesthetized for electrode implantation on the frontal and parietal skulls. EEG signals were recorded for 180 min following intraperitoneal injection of diazepam (10 mg/kg) or during continuous inhalation of lavender EO (200 μL) or distilled water (control). Fast Fourier transform was used for the analyses of EEG power spectra and sleep-wake parameters.
RESULTS
During a 30-60 min period, diazepam and lavender EO significantly increased frontal powers of 0.78-45.31 and 7.03-18.36 Hz, respectively. Both treatments also increased parietal powers with lower magnitudes of significant change. Significant increases in some frequency ranges remained until a 60-90 min period. Sleep-wake analyses also revealed that diazepam significantly reduced time spent in wake, increased time spent in non-rapid eye movement (NREM), increased episode duration of NREM, decreased numbers of wake episode and decreased rapid eye movement (REM) sleep latency. On the other hand, lavender EO only significantly decreased wake episodes and latency to REM sleep. Lavender EO inhalation reduced numbers of wake episode but maintain normal time spent in wake, NREM and REM sleeps.
CONCLUSIONS
These findings might suggest beneficial and distinct anxiolytic-like effects of lavender EO for sleep enhancing purposes.
民族药理学相关性
薰衣草精油（薰衣草精油）具有悠久的药用历史，特别是被认为具有抗焦虑和镇静特性。薰衣草精油芳香疗法已被用于自然减轻痛苦和改善失眠。越来越多的证据表明，薰衣草精油的作用与抗焦虑药物苯二氮䓬类相似。然而，与标准抗焦虑药地西泮相比，其对睡眠-觉醒和脑电图模式的影响仍有待探讨。
研究目的
本研究旨在比较薰衣草精油吸入与标准抗焦虑药地西泮对体内大鼠模型脑电图（EEG）图谱和睡眠模式的影响。
材料和方法
成年雄性 Wistar 大鼠麻醉后在前额和顶骨颅骨上植入电极。腹腔注射地西泮（10mg/kg）或连续吸入薰衣草精油（200μL）或蒸馏水（对照）后，记录 EEG 信号 180 分钟。快速傅里叶变换用于分析 EEG 功率谱和睡眠-觉醒参数。
结果
在 30-60 分钟期间，地西泮和薰衣草精油分别显著增加了 0.78-45.31 和 7.03-18.36 Hz 的额部功率。两种处理方法还增加了较小幅度的显著变化的顶叶功率。一些频率范围的显著增加一直持续到 60-90 分钟期间。睡眠-觉醒分析还表明，地西泮显著减少了清醒时间，增加了非快速眼动（NREM）时间，增加了 NREM 发作持续时间，减少了觉醒发作次数，减少了快速眼动（REM）睡眠潜伏期。另一方面，薰衣草精油仅显著减少了觉醒发作次数和 REM 睡眠潜伏期。薰衣草精油吸入减少了觉醒发作次数，但保持了清醒、NREM 和 REM 睡眠时间的正常。
结论
这些发现可能表明薰衣草精油具有有益的、独特的抗焦虑样作用，可用于增强睡眠。
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