碳化赖氨酸纳米凝胶可预防传染性支气管炎病毒。
Carbonized Lysine-Nanogels Protect against Infectious Bronchitis Virus.
机构信息
Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.
Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan.
出版信息
Int J Mol Sci. 2021 May 21;22(11):5415. doi: 10.3390/ijms22115415.
In this study, we demonstrate the synthesis of carbonized nanogels (CNGs) from an amino acid (lysine hydrochloride) using a simple pyrolysis method, resulting in effective viral inhibition properties against infectious bronchitis virus (IBV). The viral inhibition of CNGs was studied using both in vitro (bovine ephemeral fever virus (BEFV) and pseudorabies virus (PRV)) and in ovo (IBV) models, which indicated that the CNGs were able to prevent virus attachment on the cell membrane and penetration into the cell. A very low concentration of 30 μg mL was found to be effective (>98% inhibition) in IBV-infected chicken embryos. The hatching rate and pathology of IBV-infected chicken embryos were greatly improved in the presence of CNGs. CNGs with distinctive virus-neutralizing activities show great potential as a virostatic agent to prevent the spread of avian viruses and to alleviate the pathology of infected avian species.
在这项研究中，我们展示了一种简单的热解方法，从氨基酸（盐酸赖氨酸）合成碳化纳米凝胶（CNG），对传染性支气管炎病毒（IBV）具有有效的抑制作用。通过体外（牛暂时热病毒（BEFV）和伪狂犬病病毒（PRV））和鸡胚内（IBV）模型研究了 CNG 的抗病毒作用，结果表明 CNG 能够阻止病毒附着在细胞膜上和穿透细胞。在感染 IBV 的鸡胚中，发现浓度非常低的 30μg/mL （>98%抑制）有效。在 CNG 存在的情况下，感染 IBV 的鸡胚的孵化率和病理学得到了极大改善。具有独特病毒中和活性的 CNG 作为一种抗病毒剂，具有很大的潜力，可以防止禽病毒的传播，并减轻感染禽类的病理。
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