Anticholinesterase Activity of Eight Medicinal Plant Species: and Studies in the Search for Therapeutic Agents against Alzheimer's Disease.

Many Bangladeshi medicinal plants have been used to treat Alzheimer's disease and other neurodegenerative diseases. In the present study, the anticholinesterase effects of eight selected Bangladeshi medicinal plant species were investigated. Species were selected based on the traditional uses against CNS-related diseases. Extracts were prepared using a gentle cold extraction method. cholinesterase inhibitory effects were measured by Ellman's method in 96-well microplates. (Compositae) and (Menispermaceae) were found to have the highest acetylcholinesterase inhibitory (IC 150 ± 11 and 176 ± 14 g/mL, respectively) and butyrylcholinesterase inhibitory effect (IC, 297 ± 13 and 124 ± 2 g/mL, respectively). demonstrated competitive inhibition, where showed an uncompetitive inhibition mode for acetylcholinesterase. (Smilacaceae) and (Malvaceae) were also found to show moderate AChE inhibition (IC 205 ± 31 and 221 ± 2 g/mL, respectively), although no significant BChE inhibitory effect was observed for extracts from these plant species. Among others, grandiflora (Acanthaceae) and (Compositae) were found to display noticeable AChE (IC, 252 ± 22 g/mL) and BChE (IC, 314 ± 15 g/mL) inhibitory effects, respectively. Molecular docking experiment suggested that compounds 5-hydroxy-3,6,7,3',4'-pentamethoxyflavone (BL4) and kaempferol-3---L-rhamnopyranosyl-(1⟶6)--D-glucopyranoside (BL5) from bound stably to the binding groove of the AChE and BChE by hydrogen-bond interactions, respectively. Therefore, these compounds could be candidates for cholinesterase inhibitors. The present findings demonstrated that and are interesting objects for further studies aiming at future therapeutics for Alzheimer's disease.