Collinsella acetigenes sp. nov., an Anaerobic Actinobacterium Isolated from Human Feces, and Emended Description of the Genus Collinsella and Collinsella aerofaciens.

A novel actinobacterial strain, Gram-positive, anaerobic, non-motile, and rod-shaped, designated KGMB02528, was isolated from healthy human feces. Cells of strain KGMB02528 grew optimally at pH 7.0 and 37 °C and in the presence of 0% (w/v) NaCl. Based on 16S rRNA gene sequence similarity, strain KGMB04489 belonged to the genus Collinsella and was most closely related to Collinsella aerofaciens DSM 17552 (95.8%). The DNA G + C content was 58.0 mol%. The major cellular fatty acids (> 10%) were C DMA, C ALDE, C DMA, and C. The predominant end product of fermentation was acetic acid. The cell wall peptidoglycan of strain KGMB02528 contained alanine, glutamic acid, and lysine, while diaminopimelic acid was not detected. The polar lipids were composed of two unidentified phospholipids and unidentified nine glycolipids. Based on the phenotypic, chemotaxonomic, and phylogenetic properties, strain KGMB02528 represents a novel species of the genus Collinsella, for which the name Collinsella acetigenes sp. nov. is proposed. The type strain is Collinsella acetigenes KGMB02528 (= KCTC 15847 = CCUG 73987). The description of the genus Collinsella is emended to accommodate the new species.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Collinsella acetigenes KGMB02528 is MT117838. The whole-genome shotgun BioProject number is PRJNA623694 with the accession number JABBCP000000000.