体外评估益生菌罗伊氏乳杆菌对种植体周围炎微生物群的影响。
In vitro assessment of the effect of probiotic lactobacillus reuteri on peri-implantitis microflora.
机构信息
Department of Periodontology, Yenepoya Dental College, University road, Deralakatte, Mangalore, Karnataka, 575018, India.
Department of Oral and Dental Health, College of Applied Health Sciences in Arrass, Qassim University, Buraydah, Kingdom of Saudi Arabia.
出版信息
BMC Oral Health. 2021 Aug 19;21(1):408. doi: 10.1186/s12903-021-01762-2.
BACKGROUND
Probiotics affect both the development and stability of microbiota by altering the colonization of pathogens and thus helps in stimulating the immune system of the individual. The aim of the present study is to assess the effect of probiotics on peri-implantitis microflora, by determining the minimum inhibitory concentration (MIC) of Lactobacillus reuteri, that can be effectively administered as an antimicrobial agent on specific peri-implantitis pathogens. Hence, this study will be helpful in finding the MIC of L. Reuteri that can be effectively administered as an antimicrobial agent on specific peri-implantitis pathogens.
METHODS
This experimental research was conducted on patients visiting the periodontology department in M. A. Rangoonwala college of dental sciences and research centre. Sub-gingival plaque samples were collected from peri-implantitis patients to identify various peri-implantitis microorganisms. The identified microorganisms were compared to each other and Chi-Square test was used to calculate statistical significance. The isolated microorganisms were subjected to the effect of probiotic Lactobacillus reuteri in-vitro. Minimum inhibitory concentration (MIC) was assessed using serial dilution method.
RESULTS
The research results showed the presence of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Streptococcus salivaris and Staphylococcus aureus in the subgingival samples from peri-implantitis patients. Statistically, significantly higher proportion of samples had Porphyromonas gingivalis. When subjected to the effect of L. reuteri, all the microorganisms were affected by L.reuteri except Aggregatibacter actinomycetemcomitans.
CONCLUSION
This study provides the various MIC value for each isolated pathogen against L.reuteri. The authors recommend to avoid using standard guidelines for probiotic dose in the treatment of peri-implant infections as the antimicrobial profile is different for each periodontal pathogen.
背景
益生菌通过改变病原体的定植来影响微生物群落的发育和稳定性，从而帮助刺激个体的免疫系统。本研究的目的是通过确定可有效作为抗菌剂用于特定种植体周围炎病原体的罗伊氏乳杆菌最小抑菌浓度（MIC），来评估益生菌对种植体周围炎微生物群的影响。因此，这项研究将有助于找到可有效作为抗菌剂用于特定种植体周围炎病原体的 L. Reuteri 的 MIC。
方法
这项实验研究是在 M.A.Rangoonwala 牙科科学与研究中心的牙周病科就诊的患者中进行的。从种植体周围炎患者中采集龈下菌斑样本，以鉴定各种种植体周围炎微生物。将鉴定出的微生物相互比较，并使用卡方检验计算统计显著性。将分离出的微生物进行体外益生菌罗伊氏乳杆菌的作用。使用连续稀释法评估最小抑菌浓度（MIC）。
结果
研究结果表明，种植体周围炎患者龈下样本中存在牙龈卟啉单胞菌、伴放线放线杆菌、中间普氏菌、唾液链球菌和金黄色葡萄球菌。统计学上，牙龈卟啉单胞菌的样本比例显著更高。当受到 L. reuteri 的影响时，除伴放线放线杆菌外，所有微生物均受到 L.reuteri 的影响。
结论
本研究提供了每种分离病原体对 L.reuteri 的各种 MIC 值。作者建议避免在种植体周围感染的治疗中使用益生菌剂量的标准指南，因为每种牙周病原体的抗菌谱都不同。
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