Assessment the using of silica nanoparticles (SiONPs) biosynthesized from rice husks by MF780864 as water lead adsorbent for immune status of Nile tilapia (.

Rice husks (RHs) was used as a substrate for biosynthesis of high-value Silica nanoparticles (SiONPs). An isolate of MF780864 () was isolated and identified based on the Internal Transcribed Spacers (ITS) sequences; it showed the potentiality to induce SiONPs in the process of RHs biotransformation. SiONPs were produced extracellularly and their size was of about 89 nm. SiONPs characterized by oval, rod and cubical particles by using Transmission Electron Microscope (TEM).The Fourier transform infrared spectroscopy (FTIR) confirmed the presence of various functional groups of biomolecules and capping protein, encapsulating SiONPs. Water and fish samples were collected from private fish farms in El-Sharkia Governorate, Egypt. Lead (Pb) was detected from water and fish samples at its highest concentration at about 0.088 mg/L. The adsorption capacity of Pb by SiONPs was evaluated by testing different concentrations of SiONPs viz. 1, 2, and 3 mg/L, wherein 1 mg/L revealed the highest Pb adsorption efficiency. Within laboratory trials, the results indicated that highest Pb adsorption efficiency revealed through the increasing of SiONPs concentrations until 120 h. trial that lasted for 8 weeks, Nile tilapia ( (29.78 ± 0.36 g body weight) supplemented with 0.088 mg/L Pb was divided into four experimental groups having three replicates (15 fish/replicate; 45 fish/group). The results showed that SiONPs supplementation through water revealed significant increase in growth and hematological parameters of . Moreover, enhancement of antioxidant capacity (TAC), and immune related gene expression of IL-1 were increased in the presence of SiONPs compared with the groups of Pb exposure. Moreover, Pb residue level in fish muscles was noticeably decreased in the SiONPs treated groups. Thus, this research opens up other possibilities in the field of using SiONPs as a lead adsorbent for water bioremediation.