帕金森病中睑脂的脂质组成与构象
Meibum Lipid Composition and Conformation in Parkinsonism.
作者信息
Blinchevsky Solomon, Ramasubramanian Aparna, Borchman Douglas, Sayied Shanzeh, Venkatasubramanian Krithika
机构信息
Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, KY, USA.
Phoenix Children's Hospital, Phoenix, AZ, USA.
出版信息
EC Ophthalmol. 2021 Apr;12(4):20-29. Epub 2021 Mar 31.
PURPOSE
Patients with Parkinson's disease (PD) exhibit unstable tear films. Tear film lipid composition and structure are related to tear film stability and dry eye and tear lipids have not been characterized in people with PD. The aim of this study is to characterize Meibum tear lipids in donors with PD using H-NMR and infrared spectroscopy.
METHODS
Three cohorts were compared: meibum from donors with PD (Mp) n = 10, meibum from donors with PD and dry eye (Mpd) n = 3, meibum from donors without PD (Mn) n = 29.
RESULTS
There were no significant differences, P > 0.05, in hydrocarbon branching for Mp compared with Mn. Mn contained twice as much cholesteryl esters compared with Mp, P < 0.0001. The cooperativity of the phase transition was significantly 37% lower for Mp compared with Mn, P < 0.0001. Mpd was much more ordered (stiffer) with compared with Mp and Mn, P < 0.0001.
CONCLUSION
Changes in meibum lipid composition and structure could be a marker for and/or contribute to increase the susceptibility of dry eye in patients with PD. A less cooperative phase transition for Mp compared with Mn indicates that Mp was more heterogeneous and/or contained more contaminants than Mn. The data support the idea that more ordered lipid contributes to dry eye.
目的
帕金森病（PD）患者表现出泪膜不稳定。泪膜脂质成分和结构与泪膜稳定性及干眼相关，而PD患者的泪液脂质尚未得到充分表征。本研究的目的是使用氢核磁共振（H-NMR）和红外光谱对PD供体的睑脂进行表征。
方法
比较了三个队列：PD供体的睑脂（Mp），n = 10；PD合并干眼供体的睑脂（Mpd），n = 3；非PD供体的睑脂（Mn），n = 29。
结果
与Mn相比，Mp的烃链分支没有显著差异，P > 0.05。与Mp相比，Mn中的胆固醇酯含量是其两倍，P < 0.0001。与Mn相比，Mp的相变协同性显著降低37%，P < 0.0001。与Mp和Mn相比，Mpd的有序性更高（更硬），P < 0.0001。
结论
睑脂成分和结构的变化可能是PD患者干眼易感性增加的一个标志和/或促成因素。与Mn相比，Mp的相变协同性较低，表明Mp比Mn更不均匀和/或含有更多污染物。这些数据支持了更有序的脂质会导致干眼的观点。
Zotero 插件