精油的细胞毒性、抗氧化和抗菌活性的风险评估。

Risk assessment of cytotoxicity, antioxidant and antimicrobial activities of L. essential oil.

机构信息

Agroindustrial Science and Technology Department, Federal University of Pelotas, Campus Capão Do Leão, Pelotas, Brazil.

Postgraduate Program in Biochemistry and Bioprospecting - Laboratory of Neurochemistry, Inflammation and Cancer, Center for Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, University Campus S/n, Pelotas, Brazil.

出版信息

J Toxicol Environ Health A. 2022 Mar 19;85(6):230-242. doi: 10.1080/15287394.2021.1999875. Epub 2021 Nov 15.

DOI:10.1080/15287394.2021.1999875

PMID:34781835

Abstract

The objective of this study was to determine the chemical composition as well as antioxidant, antibacterial, and cytotoxic properties of the essential oil of L. (peppermint). Fifteen chemical constituents were identified in the essential oil, for a total of 99.99% of the compounds. The essential oil exhibited antimicrobial activity against two Gram-positive bacteria and . The minimum inhibitory concentration (MIC) of essential oil of L. for and was 1.84 μg/ml, whereas the minimum bactericidal concentration (MBC) values were 3.7 and 7.43 μg/ml, respectively. The oil displayed potent antioxidant activity inhibiting up to approximately73% of 2,2'-azinothiobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals. In the cytotoxicity assay, the highest essential oil concentration (100 μg/ml) resulted in viability of approximately 90% human epidermal keratinocyte (HaCaT) cells. With respect to antitumor activity in C6 rat glioma cells, there was significant reduction in cell viability: 56-74% in 24 hr, and 71-77% in 48 hr. Data suggest that in presence of the essential oil of L. antioxidant, antibacterial, antitumor and non-cytotoxic properties were noted.

摘要

本研究旨在确定薄荷精油的化学成分以及抗氧化、抗菌和细胞毒性特性。鉴定出精油中的 15 种化学成分，占化合物总量的 99.99%。精油对两种革兰氏阳性菌和表现出抗菌活性。薄荷精油对和的最小抑菌浓度（MIC）分别为 1.84μg/ml，而最小杀菌浓度（MBC）值分别为 3.7 和 7.43μg/ml。该油显示出强大的抗氧化活性，可抑制高达约 73%的 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基。在细胞毒性试验中，最高浓度的精油（100μg/ml）使人类表皮角质形成细胞（HaCaT）的活力约为 90%。就 C6 大鼠神经胶质瘤细胞的抗肿瘤活性而言，细胞活力显著降低：24 小时内为 56-74%，48 小时内为 71-77%。数据表明，在薄荷精油存在的情况下，观察到抗氧化、抗菌、抗肿瘤和非细胞毒性特性。