A novel Near-Infrared fluorescent probe for Zn and CN double detection based on dicyanoisfluorone derivatives with highly sensitive and selective, and its application in Bioimaging.

We have successfully synthesized NIRF as a near-infrared fluorescence probe for relay recognition of zinc and cyanide ions. The probe possesses well selectivity and anti-interference ability over common ions towards Zn and CN. The results showed that Zn and the probe formed [NIRF-Zn] complex after added Zn into the probe NIRF solution, which emited red fluorescence. The probe can be used for quantitative detection of Zn with a detection limit of 4.61 × 10 M. It was determined that the binding stoichiometry between the NIRF and Zn was 1:1 according to the jobs curve. Subsequently, CN was added to the NIRF-Zn solution, CN combined with Zn to generate [Zn(CN)] due to the stronger binding ability between zinc ion and cyanogen, which lead to the red fluorescence disappeared. The quantitative detection of CN was realized with a detection limit of 7.9 × 10 M. In addition, the probe has excellent specificity and selectivity for Zn and CN. And the probe can be stable in a wide range of pH. Through biological experiments, we found that it can complete cell imaging in macrophages and imaging of living mice, which has application prospects in Bioimaging. In addition, the probe NIRF has good applicability for Zn and CN detection in actual samples.