临床呼出冷凝物样本中 SARS-CoV-2 的灭活用于代谢组学分析。
Inactivation of SARS-CoV-2 in clinical exhaled breath condensate samples for metabolomic analysis.
机构信息
Department of Medical Microbiology and Immunology, School of Medicine, University of California Davis, Davis, CA 95616, United States of America.
Mechanical and Aerospace Engineering, University of California Davis, Davis, CA 95616, United States of America.
出版信息
J Breath Res. 2021 Dec 20;16(1). doi: 10.1088/1752-7163/ac3f24.
Exhaled breath condensate (EBC) is routinely collected and analyzed in breath research. Because it contains aerosol droplets, EBC samples from SARS-CoV-2 infected individuals harbor the virus and pose the threat of infectious exposure. We report for the first time a safe and consistent method to fully inactivate SARS-CoV-2 in EBC samples and make EBC samples safe for processing and analysis. EBC samples containing infectious SARS-CoV-2 were treated with several concentrations of acetonitrile. The most commonly used 10% acetonitrile treatment for EBC processing failed to completely inactivate the virus in samples and viable virus was detected by the assay of SARS-CoV-2 infection of Vero E6 cells in a biosafety level 3 laboratory. Treatment with either 50% or 90% acetonitrile was effective to completely inactivate the virus, resulting in safe, non-infectious EBC samples that can be used for metabolomic analysis. Our study provides SARS-CoV-2 inactivation protocol for the collection and processing of EBC samples in the clinical setting and for advancing to metabolic assessments in health and disease.
呼出气冷凝物 (EBC) 在呼吸研究中通常被收集和分析。由于其含有气溶胶液滴，因此来自 SARS-CoV-2 感染个体的 EBC 样本携带病毒，并具有感染暴露的威胁。我们首次报道了一种安全且一致的方法，可以使 EBC 样本中的 SARS-CoV-2 完全失活，并使 EBC 样本在处理和分析时是安全的。用几种浓度的乙腈处理含有传染性 SARS-CoV-2 的 EBC 样本。最常用于 EBC 处理的 10%乙腈处理未能使样本中的病毒完全失活，并且在生物安全 3 级实验室中通过 SARS-CoV-2 感染 Vero E6 细胞的测定检测到存活病毒。用 50%或 90%的乙腈处理是有效的，可以完全使病毒失活，从而产生安全、无传染性的 EBC 样本，可用于代谢组学分析。我们的研究为临床环境中 EBC 样本的收集和处理以及在健康和疾病中的代谢评估提供了 SARS-CoV-2 失活方案。
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