Genomic pattern analysis of field isolates by pulsed-field gel electrophoresis (PFGE) discriminatory typing.

BACKGROUND AND OBJECTIVES

Glanders is a serious zoonotic disease caused by Prevention, control, and treatment strategies of glanders are prerequisites for microbial source tracking. The present study was aimed to analyze the genomic pattern of Iranian field isolates by pulsed-field gel electrophoresis (PFGE) typing.

MATERIALS AND METHODS

isolates were aerobically cultured in nutrient broth/agar supplemented with glycerol 4% for 48 h at 37°C. API 20NE identification system was used for the biochemical characterization. Genomic DNA of bacterial isolates was extracted using OIE-recommended protocol. Molecular identification of bacterial isolates was done based on amplification of and IS407-flip genes. PFGE was applied to prepare the genomic pattern of isolates. The guinea pig was used as a suitable model for studying the histopathological characterization of .

RESULTS

In both enzymatic digestion patterns by using II and I, we found three different clonal types; І) PFGE type of Razi 325 strain, ІІ) PFGE type of Tiger, Kordan, and Oshnavieh strains, and ІІІ) PFGE type of Semirom strain. Razi 325 was categorized as unrelated strain which was belonged to the different cluster differing more than four bands.

CONCLUSION

PFGE showed more discriminatory power and considerable reproducibility for molecular typing of strains in our study. It is standardized the approaches for outbreak detection, pathogen phylogeny, molecular epidemiology, and population studies.