真菌次生代谢产物合成相关基因的靶向及异源表达研究进展
Advances in targeting and heterologous expression of genes involved in the synthesis of fungal secondary metabolites.
作者信息
Qiao Yun-Ming, Yu Rui-Lin, Zhu Ping
机构信息
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, NHC Key Laboratory of Biosynthesis of Natural Products, CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs, Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100050 China
出版信息
RSC Adv. 2019 Oct 30;9(60):35124-35134. doi: 10.1039/c9ra06908a. eCollection 2019 Oct 28.
The revolutionary discovery of penicillin only marks the start of our exploration for valuable fungal natural products. Advanced genome sequencing technologies have translated the fungal genome into a huge reservoir of "recipes" - biosynthetic gene clusters (BGCs) - for biosynthesis. Studying complex fungal genetics demands specific gene manipulation strategies. This review summarizes the current progress in efficient gene targeting in fungal cells and heterologous expression systems for expressing fungal BGCs of fungal secondary metabolites.
青霉素的革命性发现仅仅标志着我们对有价值的真菌天然产物探索的开始。先进的基因组测序技术已将真菌基因组转化为一个巨大的生物合成“配方”库——生物合成基因簇（BGCs）。研究复杂的真菌遗传学需要特定的基因操作策略。本综述总结了真菌细胞中高效基因靶向以及用于表达真菌次级代谢产物真菌BGCs的异源表达系统方面的当前进展。
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