锰对保存液保护离体猪肾功效的影响。

Effect of Manganese on the Efficacy of Preservative Solution in Protecting Isolated Porcine Kidneys.

机构信息

Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Katowice, Poland.

Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Katowice, Poland; "Biochefa" Pharmaceutical Research and Production Plant, Sosnowiec, Poland.

出版信息

Transplant Proc. 2022 May;54(4):874-877. doi: 10.1016/j.transproceed.2022.02.042. Epub 2022 May 7.

DOI:10.1016/j.transproceed.2022.02.042

PMID:35537875

Abstract

BACKGROUND

Manganese is a bioelement essential for the proper functioning of the body. Mn is a cofactor for many enzymes, including manganese superoxide dismutase. Together with the enzymes, it participates in cell defense against reactive oxygen species, immune response, and energy production in mitochondria. The aim of this study was to evaluate the effect of manganese added to Biolasol solution on the storage efficiency of isolated pig kidneys.

METHODS

Twenty kidneys from Polish Great White pigs were used in the study. Biolasol solution was modified with the addition of Mn at a concentration of 1 µg/L. Grafts were assigned to 2 groups: A/Biolasol (n = 10) and B/Biolasol + Mn (n = 10). The kidneys were washed with the developed solutions, then stored using the static method for 48h under hypothermia (4°C) and washed again. Markers of normal renal function were analyzed in the collected perfusates-aspartate aminotransferase (AST), alanine aminotransferase (ALT), protein, creatinine, urea, and electrolytes.

RESULTS

After 48 hours and 30 minutes of renal preservation, activities/concentrations of biochemical parameters in the Biolasol + Mn group increased (ALT: by 33%; AST: by 86%; urea: by 18%; creatinine: 3.25-fold [P < .05]) vs Biolasol. K ion concentration was 33% lower in the Biolasol group after 48 hours and 30 minutes (P < .05) vs group B. The presence of manganese in the composition of Biolasol solution influenced the maintenance of optimal Na ion concentration.

CONCLUSIONS

Manganese added to the Biolasol solution formulation at a dose of 1 µg/L showed low efficacy in protecting nephrons. We do not recommend adding manganese to the preservative solution formulation.

摘要

背景

锰是人体正常功能所必需的生物元素。Mn 是许多酶的辅助因子，包括锰超氧化物歧化酶。与酶一起，它参与细胞对活性氧物质、免疫反应和线粒体能量产生的防御。本研究旨在评估在 Biolasol 溶液中添加锰对分离猪肾储存效率的影响。

方法

本研究使用了 20 个来自波兰大白猪的肾脏。Biolasol 溶液中添加了浓度为 1µg/L 的 Mn。将移植物分为 2 组：A/Biolasol（n=10）和 B/Biolasol+Mn（n=10）。用开发的溶液冲洗肾脏，然后在 4°C 低温下使用静态方法储存 48 小时，再次冲洗。收集的灌流液中分析了正常肾功能的标志物-天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、蛋白质、肌酐、尿素和电解质。

结果

在肾保存 48 小时 30 分钟后，Biolasol+Mn 组的生化参数活性/浓度增加（ALT 增加 33%；AST 增加 86%；尿素增加 18%；肌酐增加 3.25 倍[P<0.05]）与 Biolasol 相比。Biolasol 组在 48 小时 30 分钟后 K 离子浓度降低 33%（P<0.05）与 B 组相比。Biolasol 溶液配方中添加的锰对维持最佳 Na 离子浓度有一定影响。