通过等离子体功能化定制 3D 打印 PEEK 医用植入物的生物响应。
Tailoring the biologic responses of 3D printed PEEK medical implants by plasma functionalization.
机构信息
Department of Prosthodontics, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, NHC Key Laboratory of Digital Technology of Stomatology, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China; University Hospital Tübingen, Section Medical Materials Science and Technology, Osianderstr. 2-8, Tübingen D-72076, Germany.
Medical Additive Manufacturing Research Group, Hightech Research Center, Department of Biomedical Engineering, University of Basel, Allschwil, Switzerland; Department of Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, Basel, Switzerland.
出版信息
Dent Mater. 2022 Jul;38(7):1083-1098. doi: 10.1016/j.dental.2022.04.026. Epub 2022 May 11.
OBJECTIVE
The objective of this study was to determine the effect of two plasma surface treatments on the biologic responses of PEEK medical implants manufactured by fused filament fabrication (FFF) 3D printing technology.
METHODS
This study created standard PEEK samples using an FFF 3D printer. After fabrication, half of the samples were polished to simulate a smooth PEEK surface. Then, argon (Ar) or oxygen (O) plasma was used to modify the bioactivity of FFF 3D printed and polished PEEK samples. Scanning electron microscopy (SEM) and a profilometer were used to determine the microstructure and roughness of the sample surfaces. The wettability of the sample surface was assessed using a drop shape analyzer (DSA) after plasma treatment and at various time points following storage in a closed environment. Cell adhesion, metabolic activity, proliferation, and osteogenic differentiation of SAOS-2 osteoblasts were evaluated to determine the in vitro osteogenic activity.
RESULTS
SEM analysis revealed that several spherical nanoscale particles and humps appeared on sample surfaces following plasma treatment. The wettability measurement demonstrated that plasma surface treatment significantly increased the surface hydrophilicity of PEEK samples, with only a slight aging effect found after 21 days. Cell adhesion, spreading, proliferation, and differentiation of SAOS-2 osteoblasts were also up-regulated after plasma treatment. Additionally, PEEK samples treated with O plasma demonstrated a higher degree of bioactivation than those treated with Ar.
SIGNIFICANCE
Plasma-modified PEEK based on FFF 3D printing technology was a feasible and prospective bone grafting material for bone/dental implants.
目的
本研究旨在确定两种等离子体表面处理方法对熔融沉积成型（FFF）3D 打印技术制造的聚醚醚酮（PEEK）医学植入物的生物学反应的影响。
方法
本研究使用 FFF 3D 打印机制造标准 PEEK 样本。制造后，将一半样本进行抛光以模拟光滑的 PEEK 表面。然后，使用氩气（Ar）或氧气（O）等离子体来修饰 FFF 3D 打印和抛光的 PEEK 样本的生物活性。使用扫描电子显微镜（SEM）和轮廓仪来确定样品表面的微观结构和粗糙度。在等离子体处理后以及在封闭环境中储存的不同时间点，使用滴形分析仪（DSA）评估样品表面的润湿性。评估 SAOS-2 成骨细胞的细胞黏附、代谢活性、增殖和成骨分化，以确定体外成骨活性。
结果
SEM 分析显示，等离子体处理后样品表面出现了几个球形纳米级颗粒和凸起。润湿性测量表明，等离子体表面处理显著提高了 PEEK 样品的表面亲水性，在 21 天后仅发现轻微的老化效应。细胞黏附、铺展、增殖和 SAOS-2 成骨细胞的分化在等离子体处理后也得到了上调。此外，与 Ar 等离子体处理相比，O 等离子体处理的 PEEK 样品具有更高的生物活性。
意义
基于 FFF 3D 打印技术的等离子体改性 PEEK 是一种可行且有前景的骨/牙科植入物用骨移植材料。
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