鲁米诺-没食子酸化学发光法用于没食子酸、钴离子和酪氨酸酶的多重检测。
Lucigenin-pyrogallol chemiluminescence for the multiple detection of pyrogallol, cobalt ion, and tyrosinase.
机构信息
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
University of Science and Technology of China, Hefei, 230000, PR China.
出版信息
J Food Drug Anal. 2021 Sep 15;29(3):510-520. doi: 10.38212/2224-6614.3361.
Developing new, cheap, sensitive and selective chemiluminescence (CL) systems with multiple detection properties is still a big challenge for biological and environmental applications. Here, we report a new CL system having multiple detection applications (environmental and biological). The developed lucigenin-pyrogallol system gave an enhancement (190 times) over the conventional lucigenin-HO CL system. Cobalt ion and tyrosinase can inhibit lucigenin-PG CL intensity. Based on these phenomena, we have developed new CL methods for the detection of pyrogallol (LOD = 0.94 μM), lucigenin (LOD = 0.42 μM), cobalt (LOD = 68 nM), and tyrosinase (LOD = 1.8 μg/mL). Furthermore, the developed system gave excellent recoveries in real samples within the range of 93.59-103.12% for the assay of pyrogallol, Co and tyrosinase in coffee, lake water, and human plasma, respectively.
开发具有多种检测性能的新型、廉价、灵敏和选择性的化学发光（CL）系统仍然是生物和环境应用的一大挑战。在这里，我们报告了一种具有多种检测应用（环境和生物）的新型 CL 系统。所开发的鲁米诺-没食子酸体系比传统的鲁米诺-HO CL 体系增强了 190 倍。钴离子和酪氨酸酶可以抑制鲁米诺-PG CL 强度。基于这些现象，我们已经开发了用于检测没食子酸（LOD = 0.94 μM）、鲁米诺（LOD = 0.42 μM）、钴（LOD = 68 nM）和酪氨酸酶（LOD = 1.8 μg/mL）的新 CL 方法。此外，该体系在咖啡、湖水和人血浆中检测没食子酸、Co 和酪氨酸酶的实际样品中，回收率在 93.59-103.12%的范围内表现出色。
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