人类基因组与严重急性呼吸综合征冠状病毒2（SARS-CoV-2）检测引物之间的核苷酸序列共享
Nucleotide Sequence Sharing between the Human Genome and Primers for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Detection.
作者信息
Kanduc Darja
机构信息
Department of Biosciences, Biotechnologies, and Biopharmaceutics, University of Bari, Bari, Italy.
出版信息
Glob Med Genet. 2022 Jun 13;9(2):182-184. doi: 10.1055/s-0042-1743260. eCollection 2022 Jun.
Abstract
This study shows that oligonucleotide sequences are shared between the human genome and primers that have been proposed/used for SARS-CoV-2 detection by polymerase chain reaction (PCR). The high level of sharing (namely, up to 19mer with a maximum number of gaps equal to 2) might bear implications for the diagnostic validity of SARS-CoV-2 detection by PCR.
摘要
本研究表明，人类基因组与已被提出/用于通过聚合酶链反应（PCR）检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的引物之间存在寡核苷酸序列共享。高度的序列共享（即长达19聚体，最大间隙数等于2）可能对通过PCR检测SARS-CoV-2的诊断有效性产生影响。
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