Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using electroporation technique.

Here, we present a detailed protocol for identification and analysis of senescent neuronal stem cells (NSCs) in the developing mouse embryonic neocortex using electroporation. We describe the procedures of electroporation and brain slide preparation. We then detail the procedures of senescence-associated β-galactosidase (SA-β-Gal) staining and immunofluorescence staining, followed by microscope imaging analysis. This combined approach can be used to study the senescence of electroporated embryonic brains. For complete details on the use and execution of this protocol, please refer to Zhu et al. (2021).