四斑按蚊和白纹伊蚊对美国东北部流行的具有遗传差异的 Jamestown Canyon 病毒株的媒介效能。
Vector competence of Anopheles quadrimaculatus and Aedes albopictus for genetically distinct Jamestown Canyon virus strains circulating in the Northeast United States.
机构信息
Institut Pasteur de Guinée, Conakry, Guinea.
Wadsworth Center, New York State Department of Health, Slingerlands, NY, USA.
出版信息
Parasit Vectors. 2022 Jun 23;15(1):226. doi: 10.1186/s13071-022-05342-3.
BACKGROUND
Jamestown Canyon virus (JCV; Peribunyaviridae, Orthobunyavirus) is a mosquito-borne pathogen belonging to the California serogroup. The virus is endemic in North America and increasingly recognized as a public health concern. In this study, we determined the vector competence of Anopheles (An.) quadrimaculatus and Aedes (Ae.) albopictus for five JCV strains belonging to the two lineages circulating in the Northeast.
METHODS
An. quadrimaculatus and Ae. albopictus were fed blood meals containing two lineage A strains and three lineage B strains. Vector competence of both mosquito species was evaluated at 7- and 14-days post-feeding (dpf) by testing for virus presence in bodies, legs, and saliva.
RESULTS
Our results demonstrated that Ae. albopictus mosquitoes are a competent vector for both lineages, with similar transmission levels for all strains tested. Variable levels of infection (46-83%) and dissemination (17-38%) were measured in An. quadrimaculatus, yet no transmission was detected for the five JCV strains evaluated.
CONCLUSIONS
Our results demonstrate that establishment of Ae. albopictus in the Northeast could increase the risk of JCV but suggest An. quadrimaculatus are not a competent vector for JCV.
背景
Jamestown Canyon 病毒（JCV；Peribunyaviridae，Orthobunyavirus）是一种由蚊子传播的病原体，属于加利福尼亚血清群。该病毒在北美流行，并越来越被认为是一个公共卫生问题。在这项研究中，我们确定了携带 JCV 的两种谱系的五种 JCV 株在两种蚊子中的媒介能力，这两种谱系在东北地区循环。
方法
用含有两种谱系 A 株和三种谱系 B 株的血液餐喂养 An. quadrimaculatus 和 Ae. albopictus。在 7 天和 14 天的喂食后（dpf）通过测试身体、腿部和唾液中病毒的存在来评估两种蚊子的媒介能力。
结果
我们的结果表明，Ae. albopictus 蚊子是两种谱系的有效媒介，所有测试的菌株都具有相似的传播水平。在 An. quadrimaculatus 中测量了不同程度的感染（46-83%）和传播（17-38%），但对评估的五种 JCV 株没有检测到传播。
结论
我们的结果表明，Ae. albopictus 在东北地区的建立可能会增加 JCV 的风险，但表明 An. quadrimaculatus 不是 JCV 的有效媒介。
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