ERK-1 和 ERK-2 基因多态性在 PCOS 发病机制中的作用。
The role of ERK-1 and ERK-2 gene polymorphisms in PCOS pathogenesis.
机构信息
Department of Reproductive Endocrinology and Infertility, Balikesir University Medical Faculty, Cagis Yerleskesi,Bigadic Yolu 17.km, 10145, Balıkesir, Turkey.
Department of Medical Biology, Pamukkale University Medical Faculty, Denizli, Turkey.
出版信息
Reprod Biol Endocrinol. 2022 Jun 29;20(1):95. doi: 10.1186/s12958-022-00967-6.
BACKGROUND
Ovulation is regulated by extracellular signal-regulated kinase-1 (ERK-1) and ERK-2 signaling mechanisms, and ERK-1/2 kinases modulates the function of most of the LH-regulated genes. Defective ERK kinase signaling that is secondary to a genetic problem contributes to both ovulatory dysfunction and metabolic problems in polycystic ovary syndrome (PCOS). We planned to investigate ERK-1 and ERK-2 gene polymorphisms in PCOS for the first time in the Turkish population.
METHODS
One hundred two PCOS patients and 102 healthy controls were recruited for this patient control study. HOMA-IR, Ferriman-Gallwey score (FGS), waist-to-hip ratio (WHR), and body mass index (BMI) were assessed. Lipid profile levels, CRP, and total testosterone were determined. ERK-2 rs2276008 (G > C) and ERK-1 rs11865228 (G > A) SNPs were analyzed with a real-time PCR system.
RESULTS
ERK-1 and ERK-2 genotypes were found to differ between the PCOS and control groups. In patients with PCOS, ERK-1 GA and ERK-2 GC genotypes were different in terms of BMI, FGS, HOMA-IR, CRP, total testosterone, and total cholesterol levels.
CONCLUSIONS
ERK-1 and ERK-2 genes are involved in PCOS pathogenesis. BMI, FGS, HOMA-IR, and CRP levels are related to the heterozygote polymorphic types of ERK-1 and ERK-2 genes.
背景
排卵受细胞外信号调节激酶-1（ERK-1）和 ERK-2 信号机制的调节，ERK-1/2 激酶调节大多数 LH 调节基因的功能。继发于遗传问题的 ERK 激酶信号传导缺陷导致多囊卵巢综合征（PCOS）的排卵功能障碍和代谢问题。我们计划首次在土耳其人群中研究 PCOS 中的 ERK-1 和 ERK-2 基因多态性。
方法
这项病例对照研究纳入了 102 例 PCOS 患者和 102 例健康对照者。评估了 HOMA-IR、Ferriman-Gallwey 评分（FGS）、腰臀比（WHR）和体重指数（BMI）。测定了血脂谱水平、CRP 和总睾酮。使用实时 PCR 系统分析了 ERK-2 rs2276008（G>C）和 ERK-1 rs11865228（G>A）SNP。
结果
ERK-1 和 ERK-2 基因型在 PCOS 和对照组之间存在差异。在 PCOS 患者中，ERK-1 GA 和 ERK-2 GC 基因型在 BMI、FGS、HOMA-IR、CRP、总睾酮和总胆固醇水平方面存在差异。
结论
ERK-1 和 ERK-2 基因参与了 PCOS 的发病机制。BMI、FGS、HOMA-IR 和 CRP 水平与 ERK-1 和 ERK-2 基因的杂合子多态类型有关。
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