CREB1 通过介导 CCNA2 和 MYOG 在牛肌母细胞中的转录来促进增殖和分化。
CREB1 promotes proliferation and differentiation by mediating the transcription of CCNA2 and MYOG in bovine myoblasts.
机构信息
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China; National Beef Cattle Improvement Center in China, Yangling, Shaanxi, PR China.
出版信息
Int J Biol Macromol. 2022 Sep 1;216:32-41. doi: 10.1016/j.ijbiomac.2022.06.177. Epub 2022 Jun 29.
The cAMP response element binding protein 1 (CREB1) is an important nuclear transcription factor in eukaryotes. To explore the potential role of CREB1 on Qinchuan bovine skeletal myoblasts, we investigated the function of CREB1 on proliferation and differentiation. In this study, we found that CREB1 promoted cell proliferation by promoting DNA synthesis in S phase and cell division in G2 phase and promoted myogenic differentiation process in bovine myoblasts. Through dual luciferase experiments, we found that CREB1 can bind to the proximal promoter regions of CCNA2 and MyoG, indicating that CREB1 can play a positive regulatory role in the proliferation and differentiation of myoblasts by mediating the transcription of CCNA2 and MyoG. In addition, through downstream target gene analysis and transcriptome sequencing, we found that CREB1 plays a role in cell proliferation, myogenic differentiation, skeletal muscle repair and other related pathways.
环磷酸腺苷反应元件结合蛋白 1（CREB1）是真核生物中一种重要的核转录因子。为了探究 CREB1 对秦川牛骨骼肌成肌细胞的潜在作用，我们研究了 CREB1 对增殖和分化的功能。在这项研究中，我们发现 CREB1 通过促进 S 期的 DNA 合成和 G2 期的细胞分裂，促进牛成肌细胞的肌生成分化过程，从而促进细胞增殖。通过双荧光素酶实验，我们发现 CREB1 可以与 CCNA2 和 MyoG 的近端启动子区域结合，表明 CREB1 可以通过介导 CCNA2 和 MyoG 的转录，在成肌细胞的增殖和分化中发挥正向调控作用。此外，通过下游靶基因分析和转录组测序，我们发现 CREB1 在细胞增殖、肌生成分化、骨骼肌修复等相关途径中发挥作用。
Zotero 插件