亚麻籽多糖改变肥胖大鼠结肠脂质代谢和能量代谢的基因表达。

Flaxseed Polysaccharide Alters Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rats.

作者信息

Wei Hua, Lin Xiaohong, Liu Liu, Peng Xichun

机构信息

College of Food Science and Engineering, Nanchang Univeristy, Nanchang 330096, China.

Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.

出版信息

Foods. 2022 Jul 5;11(13):1991. doi: 10.3390/foods11131991.

DOI:10.3390/foods11131991

PMID:35804806

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9265598/

Abstract

Obesity is one of the most serious public health challenges. Recently, we found that flaxseed polysaccharides (FPs) had an anti-obesity effect through promoting lipid metabolism, but the obesity-inhibiting pathway of FP is still unclear. In this study, after FP intervention in an obese rat model, a transcriptome study was performed to further investigate how FP intervention alters the gene expression of colonic epithelial tissues (CETs). The results showed that there were 3785 genes differentially expressed due to the FP intervention, namely 374 downregulated and 3411 upregulated genes. After analyzing all the differentially expressed genes, two classical KEGG pathways were found to be related to obesity, namely the PPAR-signaling pathway and energy metabolism, involving genes , , , , , , , , , and . QRT-PCR results showed a consistent expression trend. Our results indicate that FP promotes lipid metabolism by changing the expression of some key genes of CETs, thus inhibiting obesity.

摘要

肥胖是最严峻的公共卫生挑战之一。最近，我们发现亚麻籽多糖（FPs）通过促进脂质代谢具有抗肥胖作用，但FP的肥胖抑制途径仍不清楚。在本研究中，对肥胖大鼠模型进行FP干预后，进行了转录组研究，以进一步探究FP干预如何改变结肠上皮组织（CETs）的基因表达。结果显示，由于FP干预，有3785个基因差异表达，即374个基因下调和3411个基因上调。在分析所有差异表达基因后，发现两条经典的KEGG途径与肥胖相关，即PPAR信号通路和能量代谢，涉及基因、、、、、、、、和。QRT-PCR结果显示出一致的表达趋势。我们的结果表明，FP通过改变CETs一些关键基因的表达来促进脂质代谢，从而抑制肥胖。