Intrinsically disordered protein, DNA binding with one finger transcription factor () implicates thermotolerance in yeast and rice.

Intrinsically disorder regions or proteins (IDRs or IDPs) constitute a large subset of the eukaryotic proteome, which challenges the protein structure-function paradigm. These IDPs lack a stable tertiary structure, yet they play a crucial role in the diverse biological process of plants. This study represents the intrinsically disordered nature of a plant-specific DNA binding with one finger transcription factor (DOF-TF). Here, we have investigated the role of and characterized it as an intrinsically disordered protein. Furthermore, the molecular role of in thermal stress tolerance has been elucidated. The qRT-PCR analysis revealed that was significantly upregulated under different abiotic stress treatments in rice, particularly under heat stress. The stress-responsive transcript induction of was further correlated with enriched abiotic stress-related -regulatory elements present in its promoter region. The functional analysis of the potential role of in thermotolerance was further studied in yeast and . Ectopic expression of in yeast implicates thermotolerance response. Furthermore, the rice transgenic lines with overexpressing revealed a positive role in mitigating heat stress tolerance. Collectively, our results evidently show the intrinsically disorderedness in and its role in thermal stress response in rice.