检测澳大利亚蝙蝠物种中的丝状病毒反应性抗体。

Detection of filovirus-reactive antibodies in Australian bat species.

机构信息

CSIRO, Health and Biosecurity, Australian Centre for Disease Preparedness, Geelong, Australia.

CSIRO, Health and Biosecurity, Canberra, Australia.

出版信息

J Gen Virol. 2022 Aug;103(8). doi: 10.1099/jgv.0.001785.

DOI:10.1099/jgv.0.001785

PMID:35972225

Abstract

Bats have been implicated as the reservoir hosts of filoviruses in Africa, with serological evidence of filoviruses in various bat species identified in other countries. Here, serum samples from 190 bats, comprising 12 different species, collected in Australia were evaluated for filovirus antibodies. An in-house indirect microsphere assay to detect antibodies that cross-react with Ebola virus (; EBOV) nucleoprotein (NP) followed by an immunofluorescence assay (IFA) were used to confirm immunoreactivity to EBOV and Reston virus (; RESTV). We found 27 of 102 Yinpterochiroptera and 19 of 88 Yangochiroptera samples were positive to EBOV NP in the microsphere assay. Further testing of these NP positive samples by IFA revealed nine bat sera that showed binding to ebolavirus-infected cells. This is the first report of filovirus-reactive antibodies detected in Australian bat species and suggests that novel filoviruses may be circulating in Australian bats.

摘要

蝙蝠被认为是非洲丝状病毒的储存宿主，在其他国家的各种蝙蝠物种中也发现了丝状病毒的血清学证据。在这里，对从澳大利亚采集的 190 只蝙蝠、包括 12 个不同物种的血清样本进行了丝状病毒抗体检测。使用内部间接微球检测来检测与埃博拉病毒（EBOV）核蛋白（NP）交叉反应的抗体，然后进行免疫荧光分析（IFA），以确认对 EBOV 和 Reston 病毒（RESTV）的免疫反应性。我们发现，在微球检测中，102 只 Yinpterochiroptera 中有 27 只和 88 只 Yangochiroptera 中有 19 只对 EBOV NP 呈阳性。通过 IFA 对这些 NP 阳性样本的进一步检测显示，有 9 种蝙蝠血清与感染埃博拉病毒的细胞结合。这是首次在澳大利亚蝙蝠物种中检测到丝状病毒反应性抗体的报告，表明新型丝状病毒可能在澳大利亚蝙蝠中传播。