A co-fermentation strategy with wood hydrolysate and crude glycerol to enhance the lipid accumulation in Rhodosporidium toruloides-1588.

Wood hydrolysate has been regarded as sustainable and renewable substrate to produce microbial lipids, a potential feedstock for the biodiesel industry. Moreover, the major by-product of biofuel industries is crude glycerol but its implementation as a carbon source is still constrained due to the presence of impurities resulting in low biomass production and low lipid titer. Thus, this study investigates the effect of different carbon ratios of hydrolysate and crude glycerol on R. toruloides-1588. Hydrolysate to crude glycerol ratio of 60:40 resulted in maximum lipid accumulation of 49% (w/w), more than 90% of sugars and glycerol consumption. Further, scale-up to bench-scale fermenter resulted in 12% higher lipid accumulation (56.3% w/w, 0.15 g/L∙h) in 50% less time than flask fermentation. Hence, the ability of R. toruloides-1588 to flourish on different carbohydrates and accumulate high lipid content will be beneficial for the further development of biorefinery industries.