基于 RNA 测序的甘肃马鹿鹿角软骨 microRNAs 的鉴定。

RNA sequencing-based identification of microRNAs in the antler cartilage of Gansu red deer ().

机构信息

College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai, China.

Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai, China.

出版信息

PeerJ. 2022 Sep 21;10:e13947. doi: 10.7717/peerj.13947. eCollection 2022.

DOI:10.7717/peerj.13947

PMID:36164600

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9508884/

Abstract

BACKGROUND

The velvet antler is a complex mammalian bone organ with unique biological characteristics, such as regeneration. The rapid growth stage (RGS) is a special period in the regeneration process of velvet antler.

METHODS

To elucidate the functions of microRNAs (miRNAs) at the RGS of antler development in Gansu red deer (), we used RNA sequencing (RNA-seq) to analyze miRNA expression profiles in cartilage tissues of deer antler tips at three different growth stages.

RESULTS

The RNA-seq results revealed 1,073 known and 204 novel miRNAs, including 1,207, 1,242, and 1,204 from 30-, 60-, and 90-d antler cartilage tissues, respectively. To identify key miRNAs controlling rapid antler growth, we predicted target genes of screened 25 differentially expressed miRNAs (DEMs) and specifically expressed miRNAs (SEMs) in 60 d and annotated their functions. The KEGG results revealed that target genes of 25 DEMs and 30 SEMs were highly classified in the "Metabolic pathways", "Pathways in cancer", "Proteoglycans in cancer" and "PI3K-Akt signaling pathway". In addition, a novel miRNA (CM008039.1_315920), highly enriched in "NF-kappa B signaling pathway", may need further study.

CONCLUSIONS

The miRNAs identified in our study are potentially important in rapid antler growth. Our findings provide new insights to help elucidate the miRNA-mediated regulatory mechanisms involved during velvet antler development in .

摘要

背景

鹿茸是一种具有独特生物学特性的复杂哺乳动物骨骼器官，如再生。快速生长阶段（RGS）是鹿茸再生过程中的一个特殊时期。

方法

为了阐明甘肃马鹿（）鹿茸发育 RGS 中小分子 RNA（miRNA）的功能，我们使用 RNA 测序（RNA-seq）分析了三个不同生长阶段鹿鹿角尖端软骨组织中的 miRNA 表达谱。

结果

RNA-seq 结果显示，已知的 miRNA 有 1073 个，新的 miRNA 有 204 个，包括 30 天、60 天和 90 天的鹿茸软骨组织中的 1207、1242 和 1204 个。为了鉴定控制快速鹿茸生长的关键 miRNA，我们预测了筛选出的 25 个差异表达 miRNA（DEMs）和 60 天特异性表达 miRNA（SEMs）的靶基因，并对其功能进行了注释。KEGG 结果表明，25 个 DEM 和 30 个 SEM 的靶基因在“代谢途径”、“癌症途径”、“癌症中的蛋白聚糖”和“PI3K-Akt 信号通路”中高度分类。此外，一种在“NF-kappa B 信号通路”中高度富集的新型 miRNA（CM008039.1_315920）可能需要进一步研究。