血清和母乳中抗原特异性抗体向 疾病和 疾病高风险婴儿的母体转移证据。
Evidence of maternal transfer of antigen-specific antibodies in serum and breast milk to infants at high-risk of and disease.
机构信息
Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, WA, Australia.
Menzies School of Health Research Charles, Darwin University, Darwin, NT, Australia.
出版信息
Front Immunol. 2022 Sep 21;13:1005344. doi: 10.3389/fimmu.2022.1005344. eCollection 2022.
INTRODUCTION
Children in low-mid income countries, and First Nations children in high-income countries, experience disproportionately high rates of and infections and diseases including pneumonia and otitis media. We previously observed that infants from Papua New Guinea had no evidence of waning maternal immunity for -specific antibodies. In this study, we assessed and antibody titres in Australian First Nation mothers and infants to determine antigen-specific antibody ontogenies and whether antibody titres in infants were due to low maternal antibody titres or lack of placental transfer.
METHODS
Breast milk, infant nasopharyngeal swabs and ear assessment data were collected 1-, 2-, 7-months post-birth as well as maternal, cord and 7-month-old infant sera, from 85 Australian Aboriginal and Torres Strait Islander mother-infant pairs. Serum IgG and breast milk IgG and IgA antibody titres to antigens (PspA1, PspA2, CbpA, Ply) and antigens (PD, ChimV4, OMP26, rsPilA) were measured.
RESULTS
IgG titres in maternal and cord sera were similar for all antigens, except Ply (higher in cord; p=0.004). Sera IgG titres at 7-months of age were lower than cord sera IgG titres for all antigens (p<0.001). Infant sera IgG titres were higher than cord sera for PD (p=0.029), similar for OMP26 (p=0.817) and rsPilA (p=0.290), and lower for ChimV4 (p=0.004). Breast milk titres were similar for all antigens at 1, 2 and 7-months except OMP26 IgA (lower at 7-months than 1-month; p=0.035), PspA2 IgG (p=0.012) and Ply IgG that increased by 7-months (p=0.032). One third of infants carried nontypeable (NTHi), 45% carried 52% had otitis media (OM) observed at least once over the 7-months. 73% of infants who carried either NTHi, also had otitis media observed.
CONCLUSIONS
Similarities between maternal and cord IgG titres, and absence of waning, support a lack of maternal IgG antibodies available for cross-placental transfer. Increased maternal anti-PD IgG could offer some protection from early carriage with NTHi, and maternal immunisation strategies should be considered for passive-active immunisation of infants to protect against and diseases.
TRIAL REGISTRATION
ClinicalTrials.gov NCT00714064 and NCT00310349.
介绍
在中低收入国家，儿童和高收入国家的原住民儿童，其 感染和疾病的发生率过高，包括肺炎和中耳炎。我们之前观察到，巴布亚新几内亚的婴儿没有母体针对 特异性抗体减弱的证据。在这项研究中，我们评估了澳大利亚原住民母亲和婴儿的 和 抗体滴度，以确定抗原特异性抗体的发生情况，以及婴儿的 抗体滴度是否归因于母体抗体滴度低或胎盘转移不足。
方法
从 85 对澳大利亚土著和托雷斯海峡岛民母婴对中收集了产后 1、2、7 个月的母乳、婴儿鼻咽拭子和耳部评估数据，以及母亲、脐带和 7 个月大婴儿的血清。测量了针对 抗原（PspA1、PspA2、CbpA、Ply）和 抗原（PD、ChimV4、OMP26、rsPilA）的血清 IgG 和母乳 IgG 和 IgA 抗体滴度。
结果
除 Ply 外（脐带中较高；p=0.004），所有抗原的母体和脐带血清 IgG 滴度相似。7 月龄时婴儿血清 IgG 滴度均低于脐带血清 IgG 滴度（p<0.001）。婴儿血清 IgG 滴度高于脐带血清的 PD（p=0.029），OMP26（p=0.817）和 rsPilA（p=0.290）相似，ChimV4（p=0.004）较低。除 OMP26 IgA（7 个月时低于 1 个月；p=0.035）、PspA2 IgG（p=0.012）和 Ply IgG 增加（p=0.032）外，1、2 和 7 个月时所有抗原的母乳 IgG 滴度相似。三分之一的婴儿携带非典型性 （NTHi），45%携带 52%在 7 个月内观察到至少一次中耳炎（OM）。携带 NTHi 的婴儿中有 73%也有中耳炎。
结论
母体和脐带 IgG 滴度相似，且不存在减弱，支持母体 IgG 抗体缺乏可用于胎盘转移。增加的母体抗 PD IgG 可能为早期携带 NTHi 提供一定的保护，应考虑母体免疫接种策略，为婴儿提供被动-主动免疫，以预防 和 疾病。
试验注册
ClinicalTrials.gov NCT00714064 和 NCT00310349。
Zotero 插件