韩国红参对人冠状病毒OC43的抗病毒作用。
Antiviral effects of Korean Red Ginseng on human coronavirus OC43.
作者信息
Jeong Chi Hwan, Kim Jisu, Kim Bo Kyeong, Dan Kang Bin, Min Hyeyoung
机构信息
College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
出版信息
J Ginseng Res. 2023 Mar;47(2):329-336. doi: 10.1016/j.jgr.2022.09.009. Epub 2022 Oct 5.
BACKGROUND
is a medicinal plant well-known for its antiviral activities against various viruses, but its antiviral effect on coronavirus has not yet been studied thoroughly. The antiviral activity of Korean Red Ginseng (KRG) and ten ginsenosides against Human coronavirus OC43 (HCoV-OC43) was investigated in vitro.
METHODS
The antiviral response and mechanism of action of KRG extract and ginsenoside Rc, Re, Rf, Rg1, Rg2-20 (R) and -20 (S), Rg3-20 (R) and -20 (S), and Rh2-20 (R) and -20 (S), against the human coronavirus strain OC43 were investigated by using plaque assay, time of addition assay, real-time PCR, and FACS analysis.
RESULTS
Virus plaque formation was reduced in KRG extract-treated and HCoV-OC43-infected HCT-8 cells. KRG extract decreased the viral proteins (Nucleocapsid protein and Spike protein) and mRNA (N and M gene) expression, while increased the expression of interferon genes.
CONCLUSION
KRG extract exhibits antiviral activity by enhancing the expression of interferons and can be used in treating infections caused by HCoV-OC43.
背景
是一种以其对多种病毒的抗病毒活性而闻名的药用植物，但其对冠状病毒的抗病毒作用尚未得到充分研究。在体外研究了韩国红参（KRG）和十种人参皂苷对人冠状病毒OC43（HCoV-OC43）的抗病毒活性。
方法
采用蚀斑试验、添加时间试验、实时聚合酶链反应和荧光激活细胞分选分析，研究了KRG提取物与人参皂苷Rc、Re、Rf、Rg1、Rg2-20（R）和-20（S）、Rg3-20（R）和-20（S）以及Rh2-20（R）和-20（S）对人冠状病毒OC43株的抗病毒反应及作用机制。
结果
在KRG提取物处理并感染HCoV-OC43的HCT-8细胞中，病毒蚀斑形成减少。KRG提取物降低了病毒蛋白（核衣壳蛋白和刺突蛋白）和mRNA（N和M基因）的表达，同时增加了干扰素基因的表达。
结论
KRG提取物通过增强干扰素的表达表现出抗病毒活性，可用于治疗由HCoV-OC43引起的感染。
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