Antimicrobial Photodynamic Effect of Cross-Kingdom Microorganisms with Toluidine Blue O and Potassium Iodide.

() and () are prominent microbes associated with rapid and aggressive caries. In the present study, we investigated the antimicrobial efficacy, cytotoxicity, and mechanism of toluidine blue O (TBO)-mediated antimicrobial photodynamic therapy (aPDT) and potassium iodide (KI). The dependence of KI concentration, TBO concentration and light dose on the antimicrobial effect of aPDT plus KI was determined. The cytotoxicity of TBO-mediated aPDT plus KI was analyzed by cell counting kit-8 (CCK-8) assay. A singlet oxygen (O) probe test, time-resolved O detection, and a O quencher experiment were performed to evaluate the role of O during aPDT plus KI. The generation of iodine and hydrogen peroxide (HO) were analyzed by an iodine starch test and Amplex red assay. The anti-biofilm effect of TBO-mediated aPDT plus KI was also evaluated by counting forming unit (CFU) assay. KI could potentiate TBO-mediated aPDT against and in planktonic and biofilm states, which was safe for human dental pulp cells. O measurement showed that KI could quench O signals, implicating that O may act as a principal mediator to oxidize excess iodide ions to form iodine and HO. KI could highly potentiate TBO-mediated aPDT in eradicating and due to the synergistic effect of molecular iodine and HO.