腺嘌呤甲基化在 RNA 中的生物学作用。
Biological roles of adenine methylation in RNA.
机构信息
Division of Newborn Medicine, Boston Children's Hospital, Boston, MA, USA.
Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
出版信息
Nat Rev Genet. 2023 Mar;24(3):143-160. doi: 10.1038/s41576-022-00534-0. Epub 2022 Oct 19.
Abstract
N-Methyladenosine (mA) is one of the most abundant modifications of the epitranscriptome and is found in cellular RNAs across all kingdoms of life. Advances in detection and mapping methods have improved our understanding of the effects of mA on mRNA fate and ribosomal RNA function, and have uncovered novel functional roles in virtually every species of RNA. In this Review, we explore the latest studies revealing roles for mA-modified RNAs in chromatin architecture, transcriptional regulation and genome stability. We also summarize mA functions in biological processes such as stem-cell renewal and differentiation, brain function, immunity and cancer progression.
摘要
N6-甲基腺苷（m6A）是转录组中最丰富的修饰之一，存在于所有生命领域的细胞 RNA 中。检测和作图方法的进步提高了我们对 m6A 对 mRNA 命运和核糖体 RNA 功能影响的理解，并在几乎所有 RNA 物种中发现了新的功能作用。在这篇综述中，我们探讨了最新的研究成果，这些成果揭示了 m6A 修饰 RNA 在染色质结构、转录调控和基因组稳定性中的作用。我们还总结了 m6A 在干细胞更新和分化、大脑功能、免疫和癌症进展等生物学过程中的功能。
相似文献
1
Biological roles of adenine methylation in RNA.
Nat Rev Genet. 2023 Mar;24(3):143-160. doi: 10.1038/s41576-022-00534-0. Epub 2022 Oct 19.
2
Role of RNA methyltransferases in tissue renewal and pathology.
Curr Opin Cell Biol. 2014 Dec;31:1-7. doi: 10.1016/j.ceb.2014.06.006. Epub 2014 Jul 10.
3
Epitranscriptomics: regulation of mRNA metabolism through modifications.
Curr Opin Chem Biol. 2017 Dec;41:93-98. doi: 10.1016/j.cbpa.2017.10.008. Epub 2017 Nov 7.
4
RNA N6-methyladenosine methylation in post-transcriptional gene expression regulation.
Genes Dev. 2015 Jul 1;29(13):1343-55. doi: 10.1101/gad.262766.115.
5
The impact of epitranscriptomic marks on post-transcriptional regulation in plants.
Brief Funct Genomics. 2021 Mar 27;20(2):113-124. doi: 10.1093/bfgp/elaa021.
6
RNA m A methylation in cancer.
Mol Oncol. 2023 Feb;17(2):195-229. doi: 10.1002/1878-0261.13326. Epub 2022 Nov 6.
7
Reading, writing and erasing mRNA methylation.
Nat Rev Mol Cell Biol. 2019 Oct;20(10):608-624. doi: 10.1038/s41580-019-0168-5. Epub 2019 Sep 13.
8
Deciphering the Epitranscriptomic Signatures in Cell Fate Determination and Development.
Stem Cell Rev Rep. 2019 Aug;15(4):474-496. doi: 10.1007/s12015-019-09894-3.
9
Emerging Roles of RNA Methylation in Development.
Acc Chem Res. 2023 Dec 5;56(23):3417-3427. doi: 10.1021/acs.accounts.3c00448. Epub 2023 Nov 15.
10
Dynamic RNA modifications in posttranscriptional regulation.
Mol Cell. 2014 Oct 2;56(1):5-12. doi: 10.1016/j.molcel.2014.09.001.
引用本文的文献
1
WTAP Maintains Alternative Activation of Macrophages by Promoting IDH1-Mediated α-ketoglutarate Production.
Int J Biol Sci. 2025 Aug 22;21(12):5428-5443. doi: 10.7150/ijbs.115672. eCollection 2025.
2
mADP-GCNPUAS: mA-Disease Prediction via Graph Convolutional Network and Positive-Unlabeled Learning with Self-Adaptive Sampling.
Interdiscip Sci. 2025 Aug 30. doi: 10.1007/s12539-025-00760-0.
3
Uncovering the Epitranscriptome: A Review on mRNA Modifications and Emerging Frontiers.
Genes (Basel). 2025 Aug 12;16(8):951. doi: 10.3390/genes16080951.
4
Small-molecule and peptide inhibitors of m6A regulators.
Front Oncol. 2025 Aug 1;15:1629864. doi: 10.3389/fonc.2025.1629864. eCollection 2025.
5
SETD2 drives METTL14-mediated mA to suppress Piezo1 Attenuation and activate TGM2 to promote pulmonary hypertension.
Cell Mol Life Sci. 2025 Aug 8;82(1):302. doi: 10.1007/s00018-025-05809-3.
6
Terminal Loop Sequences in Viral Double-Stranded RNAs Modulate RIG-I Signaling.
bioRxiv. 2025 Aug 2:2025.08.02.668285. doi: 10.1101/2025.08.02.668285.
7
Nanoparticle-Delivered siRNA Targeting NSUN4 Relieves Systemic Lupus Erythematosus through Declining Mitophagy-Mediated CD8+T Cell Exhaustion.
MedComm (2020). 2025 Aug 3;6(8):e70311. doi: 10.1002/mco2.70311. eCollection 2025 Aug.
8
ALKBH1 drives tumorigenesis and drug resistance via tRNA decoding reprogramming and codon-biased translation.
Cancer Discov. 2025 Aug 4. doi: 10.1158/2159-8290.CD-24-1043.
9
Peptide Drug: Design and Clinical Applications.
MedComm (2020). 2025 Jul 25;6(8):e70287. doi: 10.1002/mco2.70287. eCollection 2025 Aug.
10
ModiDeC: a multi-RNA modification classifier for direct nanopore sequencing.
Nucleic Acids Res. 2025 Jul 19;53(14). doi: 10.1093/nar/gkaf673.
本文引用的文献
1
N-methyladenosine in poly(A) tails stabilize VSG transcripts.
Nature. 2022 Apr;604(7905):362-370. doi: 10.1038/s41586-022-04544-0. Epub 2022 Mar 30.
2
Dynamic control of chromatin-associated mA methylation regulates nascent RNA synthesis.
Mol Cell. 2022 Mar 17;82(6):1156-1168.e7. doi: 10.1016/j.molcel.2022.02.006. Epub 2022 Feb 25.
3
-methyladenosine enhances post-transcriptional gene regulation by microRNAs.
Bioinform Adv. 2022 Jan 18;2(1):vbab046. doi: 10.1093/bioadv/vbab046. eCollection 2022.
4
scDART-seq reveals distinct mA signatures and mRNA methylation heterogeneity in single cells.
Mol Cell. 2022 Feb 17;82(4):868-878.e10. doi: 10.1016/j.molcel.2021.12.038. Epub 2022 Jan 25.
5
The METTL5-TRMT112 N-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.
J Biol Chem. 2022 Mar;298(3):101590. doi: 10.1016/j.jbc.2022.101590. Epub 2022 Jan 14.
6
Dynamic regulation of N,2'-O-dimethyladenosine (mAm) in obesity.
Nat Commun. 2021 Dec 10;12(1):7185. doi: 10.1038/s41467-021-27421-2.
7
From genotype to phenotype: genetics of mammalian long non-coding RNAs in vivo.
Nat Rev Genet. 2022 Apr;23(4):229-243. doi: 10.1038/s41576-021-00427-8. Epub 2021 Nov 26.
8
Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation.
Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.
9
mA RNA methylation regulates promoter- proximal pausing of RNA polymerase II.
Mol Cell. 2021 Aug 19;81(16):3356-3367.e6. doi: 10.1016/j.molcel.2021.06.023. Epub 2021 Jul 22.
10
Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore.
Nat Biotechnol. 2021 Nov;39(11):1394-1402. doi: 10.1038/s41587-021-00949-w. Epub 2021 Jul 19.
Zotero 插件