伊拉克具有不同外壳蛋白N端片段的美人蕉黄条病毒的分子特征分析
Molecular characterization of Canna yellow streak virus with divergent coat protein N-terminal fragment in Iraq.
作者信息
Ali Ahmed K, Mamoori Yaseen I
机构信息
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
Department of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Al-Jadriya, Baghdad, Iraq.
出版信息
Mol Biol Rep. 2023 Jan;50(1):927-930. doi: 10.1007/s11033-022-08079-5. Epub 2022 Nov 10.
BACKGROUND
Canna yellow streak virus (CaYSV, family Potyviridae) infects many canna cultivars, which have been widely cultivated in the Iraqi gardens for ornamental purposes. The genetic variability within multiple genomic regions of CaYSV population has been demonstrated in the 3' part, particularly in the coat protein (CP). This work was aimed to characterize CaYSV and investigate its variability from canna plants in Iraq.
METHOD AND RESULTS
Leaves of canna plants grown in several gardens in Baghdad were tested by potyvirus group antibodies and RT-PCR. Analysis of the nucleotide (nt) sequences corresponding to the 3' part of the virus genome revealed the highest identity (81.6-90.6%) with known CaYSV isolates. Phylogenetic analysis of the coat protein (CP) gene sequence placed the Iraqi isolates in a separate clade with members of group A. This distinction was evidenced by unique amino acid changes found within the N-terminal motif of the CP. This is the first report of phylogenetically distinct CaYSV in Iraq.
CONCLUSIONS
This is the first report of phylogenetically distinct CaYSV with divergent CP N-terminus in Iraq.
背景
美人蕉黄条病毒（CaYSV，马铃薯Y病毒科）感染许多美人蕉品种，这些品种在伊拉克花园中广泛种植用于观赏。已证明CaYSV群体多个基因组区域内的遗传变异性存在于3'部分，特别是在外壳蛋白（CP）中。这项工作旨在鉴定CaYSV并研究其在伊拉克美人蕉植物中的变异性。
方法与结果
用马铃薯Y病毒属抗体和RT-PCR对在巴格达几个花园中种植的美人蕉植物叶片进行检测。对与病毒基因组3'部分相对应的核苷酸（nt）序列分析显示，与已知CaYSV分离株的同源性最高（81.6 - 90.6%）。外壳蛋白（CP）基因序列的系统发育分析将伊拉克分离株置于与A组成员单独的一个分支中。CP N端基序内发现的独特氨基酸变化证明了这种区别。这是伊拉克存在系统发育上不同的CaYSV的首次报道。
结论
这是伊拉克首次报道具有不同CP N端的系统发育上不同的CaYSV。
Zotero 插件