寻常疣中长链非编码RNA表达谱的网络分析
Network analysis of long non-coding RNA expression profiles in common warts.
作者信息
Tarkhan Amneh H, Al-Eitan Laith N, Alkhatib Rami Q, Alghamdi Mansour A
机构信息
Department of Biotechnology and Genetic Engineering, College of Science and Arts, Jordan University of Science and Technology, Irbid, 22110, Jordan.
Department of Anatomy, College of Medicine, King Khalid University, Abha, 61421, Saudi Arabia.
出版信息
Heliyon. 2022 Nov 19;8(11):e11790. doi: 10.1016/j.heliyon.2022.e11790. eCollection 2022 Nov.
BACKGROUND
Long non-coding RNAs (lncRNAs) have been the subject of considerable attention in recent years due to their role in gene regulation. However, the function of lncRNAs remains poorly understood, especially in the context of infection with low-risk human papillomaviruses (HPVs). To further understanding on this issue, we investigated lncRNA expression in HPV-induced common warts.
METHODS
A publicly available high-throughput sequencing dataset for common warts was downloaded from the Gene Expression Omnibus (GEO). lncRNA profiles were generated using the NetworkAnalyst 3.0 workflow, and a list of differentially expressed (DE) lncRNAs in common warts was identified and inputted into the ENCODE, RegNetwork, DisGeNet, and miRNet platforms.
RESULTS
A total of 54 lncRNAs were revealed to be significantly dysregulated in common warts. Of these 54 lncRNAs, 24 and 30 were upregulated and downregulated, respectively. The most significantly differentially expressed lncRNAs in common warts included the , , , and genes.
CONCLUSION
The current findings suggest that HPV-induced warts impact the host lncRNA transcriptome. To the best of our knowledge, the present study is the first to explore the impact of low-risk HPV infection on lncRNA expression profiles.
背景
长链非编码RNA（lncRNAs）近年来因其在基因调控中的作用而备受关注。然而，lncRNAs的功能仍知之甚少，尤其是在低风险人乳头瘤病毒（HPVs）感染的背景下。为了进一步了解这一问题，我们研究了HPV诱导的寻常疣中lncRNA的表达情况。
方法
从基因表达综合数据库（GEO）下载了一个公开可用的寻常疣高通量测序数据集。使用NetworkAnalyst 3.0工作流程生成lncRNA图谱，并鉴定出寻常疣中差异表达（DE）lncRNAs列表，并将其输入到ENCODE、RegNetwork、DisGeNet和miRNet平台。
结果
共发现54种lncRNAs在寻常疣中显著失调。在这54种lncRNAs中，分别有24种上调和30种下调。寻常疣中差异表达最显著的lncRNAs包括 、 、 和 基因。
结论
目前的研究结果表明，HPV诱导的疣会影响宿主lncRNA转录组。据我们所知，本研究是首次探索低风险HPV感染对lncRNA表达谱的影响。
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