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抗氧化和抗糖尿病活性提取物的比较评价。

comparative evaluation of extracts for antioxidant and antidiabetic activity.

机构信息

Department of Eastern Medicine, Government College University Faisalabad, Faisalabad 38040, Pakistan.

Faculty of Medical Sciences, Government College University Faisalabad, Faisalabad 38040, Pakistan.

出版信息

Exp Biol Med (Maywood). 2023 Feb;248(3):253-262. doi: 10.1177/15353702221139208. Epub 2022 Dec 28.

Abstract

known as Jhau is traditionally used as expectorant, liver tonic, laxative, astringent, and antidiarrheal. The current study was proposed to determine the antioxidant, antidiabetic properties of the methanolic, ethanolic, and aqueous extracts of arial part, subsequently the phytochemical evaluation. Hence, arial part extracts were extracted with methanol (MthTg), ethanol (EthTg), and distilled water (AqTg). Extracts phytochemical analysis were accomplished to identify the phenolic components (TPC and TFC). Extracts antioxidant property was evaluated by DPPH, FRAP, and ABTS assay. For antidiabetic property, α-amylase and α-glucosidase inhibitory activities were assessed. One Way ANOVA was applied statistically by means of software SPSS Statistics 23 and attained data were definite as mean ± standard deviation. Result revealed that EthTg have the maximum TPC and TFC levels than MthTg and AqTg. Antioxidant property in relations of DPPH (lowest IC = 1.309 ± 0.31), FRAP (323.51 ± 2.32), and ABTS (266.97 ± 25.14) assay was also highest in EthTg. EthTg was also exposed highest α-amylase and α-glucosidase inhibition activity with lower IC (1.116 ± 0.051; 0.402 ± 0.2, respectively). The extracts antioxidant and antidiabetic activities order was as EthTg > MthTg > AqTg. TFC and TPC also revealed directly proportional correlation with antioxidant, and antidiabetic properties of the arial part extracts. Results noticeably stated that the ethanolic extract of have the highest antioxidant and antidiabetic properties. has competency to reduce the oxidative stress and can be utilized in the management of diabetes.

摘要

名为 Jhau 的传统药物被用作祛痰剂、肝脏滋补剂、泻药、收敛剂和止泻药。本研究旨在确定其甲醇、乙醇和水提物的抗氧化、抗糖尿病特性,随后进行植物化学评价。因此,对其地上部分提取物进行了提取,分别用甲醇(MthTg)、乙醇(EthTg)和蒸馏水(AqTg)提取。通过测定总酚含量(TPC 和 TFC)对提取物的植物化学成分进行分析。通过 DPPH、FRAP 和 ABTS 测定法评估提取物的抗氧化性能。评估抗糖尿病特性时,测定了α-淀粉酶和α-葡萄糖苷酶抑制活性。通过 SPSS Statistics 23 软件进行了单因素方差分析,所得数据用平均值±标准差表示。结果表明,EthTg 的 TPC 和 TFC 水平均高于 MthTg 和 AqTg。在 DPPH(最低 IC = 1.309 ± 0.31)、FRAP(323.51 ± 2.32)和 ABTS(266.97 ± 25.14)测定中,EthTg 的抗氧化性能也是最高的。EthTg 对α-淀粉酶和α-葡萄糖苷酶抑制活性最高,IC 值分别为 1.116 ± 0.051 和 0.402 ± 0.2。提取物的抗氧化和抗糖尿病活性顺序为 EthTg > MthTg > AqTg。TFC 和 TPC 与抗氧化和抗糖尿病特性呈正相关。结果表明,该植物的乙醇提取物具有最高的抗氧化和抗糖尿病特性。具有降低氧化应激的能力,可用于糖尿病的治疗。

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